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• W63954780 abstract "Application of enzymes as feed additives is common in the livestock industry, especiallyin poultry, to eliminate the antinutritional factors present in the diets of chickens.However, the efficiency of enzymes seldom achieves their desired effects because ofdestruction during feed processing and unsuitable conditions in the gastrointestinal tract.Thus, in the present study, investigations were carried out to evaluate the potential of 12Lactobacillus strains as delivery vehicles for a heterologous β-glucanase enzyme inpoultry. The 12 Lactobacillus strains used were L. crispatus I12, L. acidophilus I16 andI26, L. fermentum I24, I25, C16 and C17, and L. brevis I23, I211, I218, C1 and C10.The strains were found to exhibit resistance to chloromphenicol, erythromycin andtetracycline in varying degrees. The erythromycin resistance of L. acidophilus I16 andI26, and L. fermentum I24 and C17 could be cured by using novobiocin, and L. brevisC10 cured by using acriflavin. The chloromphenicol and tetracycline resistances of allthe resistant strains were not eliminated even after prolonged curing in sublethalconcentrations of individual or mixtures of curing agents such as novobiocin, ethidiumbromide, acriflavin or SDS. Electrotransformation efficiency of the Lactobacillus strainswas affected by growth phase, growth and recovery medium, cell density, electroporationbuffer, buffer strength, plasmid concentration and electrical pulse. At optimizedconditions, the strains were transformed at 103-104 transformants/μg plasmid DNA. Theerythromycin susceptible wild-type strains (L. crispatus I12, L. brevis I23, I211 and I218,and L. fermentum I25) and cured derivatives (L. acidophilus I16C and I26C, L. brevisvC10C, and L. fermentum I24C and C17C) were then transformed at optimized conditionswith plasmid pSA3b6, which carried a β-glucanase gene from Bacillusamyloliquefaciens. Five wild-type Lactobacillus strains, namely, L. crispatus I12, L.fermentum I25, L. brevis I23, I211 and I218 and a cured derivative, L. brevis C10C,which could retain the plasmid at a comparatively higher rate, were used for subsequentstudies. The Lactobacillus transformants were found to secrete 32-52 U/ml of β-glucanase. Optimum activity of the enzyme was at 39 oC and pH 5-6. A loss of 0.4-1.6U/generation of β-glucanase was observed when the strains were grown under nonselectivepressure.PCR analyses of gastrointestinal samples of chickens fed transformed Lactobacillusstrains revealed that the strains could not persist for more than 24 h in the gut. The β-glucanase activity detected in the jejunum and ileum of chickens fed transformedLactobacillus strains was found to be 2-9.4 folds higher than those obtained from otherintestinal sites. In the feeding trial, supplementation of transformed Lactobacillus strainsto chickens significantly (P<0.05) improved the body weight by 2.5 %, and the feedconversion ratio by 1.0-2.6 %. In addition, the apparent metabolizable energy,digestibilities of crude protein and dry matter of feed were improved by 3.4 %, 5.9 % and3.5 %, respectively. The intestinal fluid viscosity was reduced by 21-46 %. The relativeweights of organs and intestinal segments (pancrease, liver, duodenum, jejunum, ileum,cecum and colon) were also reduced by 6-27 %, and the relative length of intestinalsegments (duodenum, jejunum, ileum and cecum) was reduced by 8-15 %. Histologicalexamination of the intestinal tissues showed that the jejunal villus height of chickens feddiet supplemented with transformed Lactobacillus strains was significantly (P<0.05)higher than those of chickens fed other dietary treatments. The transformedLactobacillus strains were also found to reduce the time of feed passage rate by 2.2 h.viThe results of the present study showed that the Lactobacillus strains have the potential tobe used as delivery vehicles for a heterologous β-glucanase enzyme in poultry." @default.
• W63954780 created "2016-06-24" @default.
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• W63954780 date "2004-03-01" @default.
• W63954780 modified "2023-09-23" @default.
• W63954780 title "Manipulation of Lactobacillus Probiotic Strains to Produce Heterologous Beta-Glucanase for Chickens" @default.
• W63954780 hasPublicationYear "2004" @default.
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