Matches in SemOpenAlex for { <https://semopenalex.org/work/W64247116> ?p ?o ?g. }
- W64247116 abstract "Traumatic central nervous system (CNS) injuries lack effective treatment options and typically result in irrecoverable tissue damage and lifelong neurologic impairment. An ideal therapeutic would provide structural support for axonal regrowth as well as modulate the default secondary injury associated with CNS injuries. Extracellular matrix (ECM) bioscaffolds derived by decellularization promoted functional remodeling in numerous non-CNS applications; however, there has been minimal investigation of this technology in the CNS. The objectives of this work were to evaluate the tissue specific properties of CNS-ECM in terms of (1) hydrogel characteristics and biochemical composition, (2) neurotrophic potential, and (3) ability to alter the innate immune response.Bioscaffolds composed of CNS-ECM were formed into injectable solutions that polymerize to form hydrogels at body temperature. Hydrogels derived from CNS-ECM were compared to a hydrogel form of a non-CNS ECM, urinary bladder matrix (UBM-ECM), using compositional analyses for retained ECM molecules, mechanical assessments for rheological and turbidimetric properties, and multiphoton microscopy to visualize in-vitro three-dimensional neurite outgrowth. ECM hydrogels from both tissue sources had mechanical properties similar to native CNS and supported three-dimensional neurite outgrowth. CNS-ECM and UBM-ECM bioscaffold mediated alteration of the innate immune and neural stem cell response was interrogated in-vitro using macrophages and spinal cord stem cells (SPCs). While all ECM scaffolds evaluated decreased astrocyte differentiation, only UBM-ECM increased SPC neuronal differentiation. Bioscaffolds derived from both CNS and non-CNS tissue sources promoted a pro-repair macrophage phenotype as demonstrated through immunofluorescent results. Finally, CNS-ECM bioscaffolds were compared to UBM-ECM in a rat model of contusion spinal cord injury. Macrophage polarization was evaluated over 4 weeks and a histologic evaluation of the lesion site completed. While the ECM bioscaffolds did not improve functional recovery, pro-repair macrophages were found closely associated with the ECM injection sites.This body of work demonstrates CNS-ECM bioscaffolds can be isolated and are capable of minimally invasive injection, supporting neurite extension in-vitro, and modulating macrophage and stem cell responses. Future research is necessary to determine the added benefits that can be obtained when this technology is combined with others known to be beneficial for CNS tissue repair." @default.
- W64247116 created "2016-06-24" @default.
- W64247116 creator A5047373881 @default.
- W64247116 date "2014-03-13" @default.
- W64247116 modified "2023-09-27" @default.
- W64247116 title "Central Nervous System Extracellular Matrix as a Therapeutic Bioscaffold for Central Nervous System Injury" @default.
- W64247116 cites W146312067 @default.
- W64247116 cites W1497283905 @default.
- W64247116 cites W1509541757 @default.
- W64247116 cites W1530777667 @default.
- W64247116 cites W1530953794 @default.
- W64247116 cites W1534398269 @default.
- W64247116 cites W1575617070 @default.
- W64247116 cites W1578985368 @default.
- W64247116 cites W1593485386 @default.
- W64247116 cites W1705218924 @default.
- W64247116 cites W1729621315 @default.
- W64247116 cites W1797422316 @default.
- W64247116 cites W18329706 @default.
- W64247116 cites W1904869986 @default.
- W64247116 cites W1942708553 @default.
- W64247116 cites W1946522955 @default.
- W64247116 cites W1964491190 @default.
- W64247116 cites W1967171619 @default.
- W64247116 cites W1967449390 @default.
- W64247116 cites W1970587959 @default.
- W64247116 cites W1971189453 @default.
- W64247116 cites W1971264738 @default.
- W64247116 cites W1973151111 @default.
- W64247116 cites W1973772247 @default.
- W64247116 cites W1974077067 @default.
- W64247116 cites W1974293368 @default.
- W64247116 cites W1974452230 @default.
- W64247116 cites W1977213877 @default.
- W64247116 cites W1979462929 @default.
- W64247116 cites W1981305966 @default.
- W64247116 cites W1981878672 @default.
- W64247116 cites W1985095303 @default.
- W64247116 cites W1985281769 @default.
- W64247116 cites W1986231376 @default.
- W64247116 cites W1986913334 @default.
- W64247116 cites W1987350026 @default.
- W64247116 cites W1988304369 @default.
- W64247116 cites W1988575276 @default.
- W64247116 cites W1988843437 @default.
- W64247116 cites W1988997888 @default.
- W64247116 cites W1990176414 @default.
- W64247116 cites W1991739120 @default.
- W64247116 cites W1991855175 @default.
- W64247116 cites W1993965974 @default.
- W64247116 cites W1994232607 @default.
- W64247116 cites W1995373597 @default.
- W64247116 cites W1996100790 @default.
- W64247116 cites W1996795547 @default.
- W64247116 cites W1996914913 @default.
- W64247116 cites W1997547509 @default.
- W64247116 cites W1998079904 @default.
- W64247116 cites W1999579326 @default.
- W64247116 cites W1999751136 @default.
- W64247116 cites W2002843301 @default.
- W64247116 cites W2003206650 @default.
- W64247116 cites W2003687276 @default.
- W64247116 cites W2005324317 @default.
- W64247116 cites W2005920106 @default.
- W64247116 cites W2006517495 @default.
- W64247116 cites W2008488746 @default.
- W64247116 cites W2009554780 @default.
- W64247116 cites W2011590786 @default.
- W64247116 cites W2011815863 @default.
- W64247116 cites W2014165538 @default.
- W64247116 cites W2015656798 @default.
- W64247116 cites W2015684586 @default.
- W64247116 cites W2018654772 @default.
- W64247116 cites W2023053568 @default.
- W64247116 cites W2026031297 @default.
- W64247116 cites W2028840661 @default.
- W64247116 cites W2030077110 @default.
- W64247116 cites W2031013234 @default.
- W64247116 cites W2032386600 @default.
- W64247116 cites W2033087883 @default.
- W64247116 cites W2033817116 @default.
- W64247116 cites W2034188706 @default.
- W64247116 cites W2037584098 @default.
- W64247116 cites W2038280189 @default.
- W64247116 cites W2038427823 @default.
- W64247116 cites W2039334993 @default.
- W64247116 cites W2041180426 @default.
- W64247116 cites W2042022714 @default.
- W64247116 cites W2042308100 @default.
- W64247116 cites W2042569529 @default.
- W64247116 cites W2043590415 @default.
- W64247116 cites W2044219916 @default.
- W64247116 cites W2045603350 @default.
- W64247116 cites W2046835548 @default.
- W64247116 cites W2047369546 @default.
- W64247116 cites W2048259921 @default.
- W64247116 cites W2048735280 @default.
- W64247116 cites W2050382572 @default.
- W64247116 cites W2050483503 @default.
- W64247116 cites W2052027207 @default.