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- W64271776 abstract "ABSTRACT Glutamine is a key metabolite for the growth of mammalian cell-lines, but it is also a relatively unstable amino acid and decomposes to produce ammonia. In order to optimize bioreactor processes a comparative study has been made on the growth of two different recombinant CH0 cell-lines in serum free medium containing various derivatives of glutamine. Investigations have been carried out both in spinner culture experiments and during continuous perfusion culture in bioreactors. The dipeptide Glycyl Glutamine was found to be an adequate substitute for glutamine, and is known to be stable over prolonged time periods. The growth and metabolism of recombinant CH0 cell-lines in medium substituted with Glycyl Glutamine will be discussed, in particular to the utilisation of amino acids and production of ammonia." @default.
- W64271776 created "2016-06-24" @default.
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- W64271776 date "1992-01-01" @default.
- W64271776 modified "2023-09-26" @default.
- W64271776 title "GROWTH AND METABOLISM OF RECOMBINANT CHO CELL-LINES IN SERUM FREE MEDIUM CONTAINING DERIVATIVES OF GLUTAMINE" @default.
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- W64271776 doi "https://doi.org/10.1016/b978-0-7506-0421-5.50050-7" @default.
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