FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W643350822> ?p ?o ?g. }

• W643350822 endingPage "363" @default.
• W643350822 startingPage "356" @default.
• W643350822 abstract "Leonotis nepetifolia (L) R. Br., Lamiaceae, a pantropical shrub, popularly known in Brazil as cordão-de-frade, rubim, is reportedly used in Brazilian ethnomedicine as well as in different countries in the treatments of ailments such as infections, inflammations, wounds, stomach disorders, among others.To evaluate its potential cytotoxicity and antibacterial mode of action of the hydroethanolic extract of L. nepetifolia (HELn) leaves, including phytochemical analysis.The cytotoxicity of HELn was investigated by Alamar blue assay, using CHO-K1 cells. Antibacterial activity of HELn was tested by broth microdilution methods against a panel of bacteria of clinical interest. The mode of action of L. nepetifolia was studied by targeting bacterial membranes. Phytochemical analysis was performed by determining total secondary metabolites with spectrophotometric assays and HPLC.HELn is not cytotoxic in the in vitro evaluation (IC50>200 μg/mL). It demonstrated a good spectrum of antibacterial activity with major activity against Shigella flexneri, Enterococcus faecalis, Staphylococcus aureus and Bacillus subtilis with MIC=6.25 µg/mL, Helicobacter pylori with MIC of 25 µg/mL and Streptococcus pyogenes with MIC of 50 µg/mL. Its mode of action is associated, at least partly, with changes in the permeability of bacterial membranes, as evidenced by the increased entry of hydrophobic antibiotics in Shigella flexneri and intense efflux of K(+) and nucleotide leakage in E. faecalis and Shigella flexneri. In addition, the presence of phenols, flavonoids and carotenoids, described in the literature to possess antibacterial effects, were detected in the composition of HELn, with high phenol content (11.55%), especially the flavonoids (6.47%).The results indicate that HELn has low cytotoxicity and potent antibacterial activity. It is bacteriostatic in nature, possibly acting at the level of bacterial membranes, especially on the cytoplasmic membrane and outer membrane, thus supporting its popular use in infectious processes. In addition, the presence of phenols, flavonoids, carotenoids, fatty acids and steroids, described in the literature as possessing antimicrobial activity, were detected in the composition of HELn." @default.
• W643350822 created "2016-06-24" @default.
• W643350822 creator A5000906292 @default.
• W643350822 creator A5005258299 @default.
• W643350822 creator A5017197011 @default.
• W643350822 creator A5018522448 @default.
• W643350822 creator A5029513051 @default.
• W643350822 creator A5030912386 @default.
• W643350822 creator A5032949618 @default.
• W643350822 creator A5034769252 @default.
• W643350822 creator A5062429259 @default.
• W643350822 creator A5073763216 @default.
• W643350822 creator A5082134641 @default.
• W643350822 creator A5086829551 @default.
• W643350822 date "2015-08-01" @default.
• W643350822 modified "2023-09-27" @default.
• W643350822 title "Antibacterial mode of action of the hydroethanolic extract of Leonotis nepetifolia (L.) R. Br. involves bacterial membrane perturbations" @default.
• W643350822 cites W1973280668 @default.
• W643350822 cites W1976780773 @default.
• W643350822 cites W1977748675 @default.
• W643350822 cites W1981445710 @default.
• W643350822 cites W1983112540 @default.
• W643350822 cites W1985163865 @default.
• W643350822 cites W1986825499 @default.
• W643350822 cites W1987940927 @default.
• W643350822 cites W1996338768 @default.
• W643350822 cites W2013013443 @default.
• W643350822 cites W2018872218 @default.
• W643350822 cites W2032901850 @default.
• W643350822 cites W2033853533 @default.
• W643350822 cites W2040839095 @default.
• W643350822 cites W2042330524 @default.
• W643350822 cites W2043449701 @default.
• W643350822 cites W2052236994 @default.
• W643350822 cites W2054661534 @default.
• W643350822 cites W2060609144 @default.
• W643350822 cites W2071952696 @default.
• W643350822 cites W2074704380 @default.
• W643350822 cites W2077099528 @default.
• W643350822 cites W2079217831 @default.
• W643350822 cites W2079339594 @default.
• W643350822 cites W2087809700 @default.
• W643350822 cites W2108329693 @default.
• W643350822 cites W2114790497 @default.
• W643350822 cites W2118970688 @default.
• W643350822 cites W2125398266 @default.
• W643350822 cites W2134456907 @default.
• W643350822 cites W2136689145 @default.
• W643350822 cites W2143006671 @default.
• W643350822 cites W2143894608 @default.
• W643350822 cites W2150324881 @default.
• W643350822 cites W2159355055 @default.
• W643350822 cites W2327648788 @default.
• W643350822 cites W2333946802 @default.
• W643350822 cites W2335220314 @default.
• W643350822 cites W4249304889 @default.
• W643350822 doi "https://doi.org/10.1016/j.jep.2015.06.027" @default.
• W643350822 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26102550" @default.
• W643350822 hasPublicationYear "2015" @default.
• W643350822 type Work @default.
• W643350822 sameAs 643350822 @default.
• W643350822 citedByCount "27" @default.
• W643350822 countsByYear W6433508222016 @default.
• W643350822 countsByYear W6433508222018 @default.
• W643350822 countsByYear W6433508222019 @default.
• W643350822 countsByYear W6433508222020 @default.
• W643350822 countsByYear W6433508222021 @default.
• W643350822 countsByYear W6433508222022 @default.
• W643350822 crossrefType "journal-article" @default.
• W643350822 hasAuthorship W643350822A5000906292 @default.
• W643350822 hasAuthorship W643350822A5005258299 @default.
• W643350822 hasAuthorship W643350822A5017197011 @default.
• W643350822 hasAuthorship W643350822A5018522448 @default.
• W643350822 hasAuthorship W643350822A5029513051 @default.
• W643350822 hasAuthorship W643350822A5030912386 @default.
• W643350822 hasAuthorship W643350822A5032949618 @default.
• W643350822 hasAuthorship W643350822A5034769252 @default.
• W643350822 hasAuthorship W643350822A5062429259 @default.
• W643350822 hasAuthorship W643350822A5073763216 @default.
• W643350822 hasAuthorship W643350822A5082134641 @default.
• W643350822 hasAuthorship W643350822A5086829551 @default.
• W643350822 hasConcept C104317684 @default.
• W643350822 hasConcept C121587506 @default.
• W643350822 hasConcept C176947019 @default.
• W643350822 hasConcept C185592680 @default.
• W643350822 hasConcept C195475562 @default.
• W643350822 hasConcept C202751555 @default.
• W643350822 hasConcept C2776635100 @default.
• W643350822 hasConcept C2779489039 @default.
• W643350822 hasConcept C2779895986 @default.
• W643350822 hasConcept C2780104969 @default.
• W643350822 hasConcept C2780950330 @default.
• W643350822 hasConcept C2781217973 @default.
• W643350822 hasConcept C3019249092 @default.
• W643350822 hasConcept C4937899 @default.
• W643350822 hasConcept C501593827 @default.
• W643350822 hasConcept C523546767 @default.
• W643350822 hasConcept C54355233 @default.

• next