Matches in SemOpenAlex for { <https://semopenalex.org/work/W644474191> ?p ?o ?g. }
- W644474191 abstract "To examine conjugal transfer of UnQlG in the soil environment, matings were performed in a laboratory soil microcosm. In sterile soil, Tn916 was observed to transfer from Bacillus subtilis to Bacillus thuringiensis subsp. israelesis at an average frequency of 2.1 x 10 ® conjugants per donor. Moreover, transfer was observed from Enterococcus faecalis to B. thuringiensis at an average frequency of 2.3 x 10 ®. In nonsterile soil, similar transfer frequencies were observed. The conjugal transfer of Tn9i6 between Bacillus species was evaluated over varying conditions of soil moisture, temperature, pH and available nutrients. Optimal transfer occurred at 2-5% moisture even though transfer was observed over the entire range tested. Detectable transfer only occurred between 18° and 37° C (optimimi at 30° C) and in a range of pH of 6 8.9; within this range, pH had minimal effect on transfer. Increasing the nutrient level in the soil enhanced transfer frequencies. Additionally, TnSifi mobilization of the nonconjugative plasmid pC194 occurred at an average frequency of 2.0 x 10 ®. Because Tn9ifi was originally isolated from E. faecalis, swine lot effluent was examined for the presence of Tn9i6-like elements. Outflow samples from the Iowa State University farrowing house were used to isolate E. faecalis. Of the enterococci isolated, 71% were found to be resistant to tetracycline. To screen these isolates for conjugal activity, a microassay was developed. Among the tetracyclineresistant enterococci isolated from the outflow sample, 34% were able to transfer tetracycline resistance to a B. thuringiensis recipient in microassay matings. The transfer frequencies for 10 random isolates were similar to those for transfer of Tn926 from E. faecalis to B. thuringiensis on filters, as weU as in a soil" @default.
- W644474191 created "2016-06-24" @default.
- W644474191 creator A5077316217 @default.
- W644474191 date "2018-08-13" @default.
- W644474191 modified "2023-09-27" @default.
- W644474191 title "The Tn916 family of conjugative transposons: their introduction and function in a soil environment" @default.
- W644474191 cites W126896380 @default.
- W644474191 cites W139682926 @default.
- W644474191 cites W1491292520 @default.
- W644474191 cites W1503958926 @default.
- W644474191 cites W1508713472 @default.
- W644474191 cites W1517681861 @default.
- W644474191 cites W1528396799 @default.
- W644474191 cites W1539599731 @default.
- W644474191 cites W1549796636 @default.
- W644474191 cites W1558647939 @default.
- W644474191 cites W156648096 @default.
- W644474191 cites W1571934585 @default.
- W644474191 cites W1572410004 @default.
- W644474191 cites W1580604153 @default.
- W644474191 cites W1587890895 @default.
- W644474191 cites W1597851536 @default.
- W644474191 cites W1647017434 @default.
- W644474191 cites W1745838399 @default.
- W644474191 cites W1795059262 @default.
- W644474191 cites W1805287899 @default.
- W644474191 cites W1819542925 @default.
- W644474191 cites W1823182538 @default.
- W644474191 cites W182721462 @default.
- W644474191 cites W1874972419 @default.
- W644474191 cites W1898485134 @default.
- W644474191 cites W1957227160 @default.
- W644474191 cites W1968485977 @default.
- W644474191 cites W1972260473 @default.
- W644474191 cites W1972590023 @default.
- W644474191 cites W1981400801 @default.
- W644474191 cites W1981617064 @default.
- W644474191 cites W1982847299 @default.
- W644474191 cites W1984637730 @default.
- W644474191 cites W1984820499 @default.
- W644474191 cites W1987349178 @default.
- W644474191 cites W1988711630 @default.
- W644474191 cites W1995136541 @default.
- W644474191 cites W2000808927 @default.
- W644474191 cites W2003338531 @default.
- W644474191 cites W2020685763 @default.
- W644474191 cites W2022035303 @default.
- W644474191 cites W2023985496 @default.
- W644474191 cites W2028088122 @default.
- W644474191 cites W2031232438 @default.
- W644474191 cites W2042437365 @default.
- W644474191 cites W2049809836 @default.
- W644474191 cites W2057516390 @default.
- W644474191 cites W2058688042 @default.
- W644474191 cites W2063450941 @default.
- W644474191 cites W2065863441 @default.
- W644474191 cites W2076683150 @default.
- W644474191 cites W2077714549 @default.
- W644474191 cites W2078556545 @default.
- W644474191 cites W2082476325 @default.
- W644474191 cites W2082855840 @default.
- W644474191 cites W2084267545 @default.
- W644474191 cites W2087643520 @default.
- W644474191 cites W2088734027 @default.
- W644474191 cites W2093436462 @default.
- W644474191 cites W2103274496 @default.
- W644474191 cites W2108776510 @default.
- W644474191 cites W2111967029 @default.
- W644474191 cites W2116396009 @default.
- W644474191 cites W2121162127 @default.
- W644474191 cites W2144634347 @default.
- W644474191 cites W2146713140 @default.
- W644474191 cites W2147208616 @default.
- W644474191 cites W2155515147 @default.
- W644474191 cites W2159651115 @default.
- W644474191 cites W2161437884 @default.
- W644474191 cites W2162825747 @default.
- W644474191 cites W2176670191 @default.
- W644474191 cites W2187252500 @default.
- W644474191 cites W2260651433 @default.
- W644474191 cites W2326592804 @default.
- W644474191 cites W2462353544 @default.
- W644474191 cites W2479013839 @default.
- W644474191 cites W2499859564 @default.
- W644474191 cites W25281428 @default.
- W644474191 cites W2586251338 @default.
- W644474191 cites W45306777 @default.
- W644474191 cites W66369755 @default.
- W644474191 cites W2396802388 @default.
- W644474191 cites W2502523056 @default.
- W644474191 doi "https://doi.org/10.31274/rtd-180813-11044" @default.
- W644474191 hasPublicationYear "2018" @default.
- W644474191 type Work @default.
- W644474191 sameAs 644474191 @default.
- W644474191 citedByCount "0" @default.
- W644474191 crossrefType "dissertation" @default.
- W644474191 hasAuthorship W644474191A5077316217 @default.
- W644474191 hasBestOaLocation W6444741911 @default.
- W644474191 hasConcept C104317684 @default.
- W644474191 hasConcept C14036430 @default.