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- W646578508 abstract "In this article we examine the limitations and potential of T-RFLP for the accurate detection of fungal species in dead wood. We collected cross-sections of decayed logs to evaluate the number of fungal species domains that are likely to be hit when drilling a sawdust sample from a log. We used these estimates to simulate the number of species that would be found using a certain number of samples. We found that in 99% of the simulations, 4 or fewer species would be contained in a sample. Based on these results we tested the probability of detecting two species of wood-decaying basidiomycetes at three different DNA concentration ratios: 1:1, 1:5 and 1:20. An additional experiment was done with 3-5 species. It was possible to detect all species at ratios higher than 1:20 but lower than 1:5; in this range all peaks were easily detected. We were able to detect all species in the mixtures of 3-5 species, with extracts from both pure cultures and wood." @default.
- W646578508 created "2016-06-24" @default.
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- W646578508 date "2014-01-01" @default.
- W646578508 modified "2023-09-26" @default.
- W646578508 title "Testing the probability of finding major decomposing basidiomycetes in logs with T-RFLP - implications for field sampling" @default.
- W646578508 hasPublicationYear "2014" @default.
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