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- W647613868 abstract "The loop-mediated isothermal amplification (LAMP) assay with high sensitivity and specificity for the detection of Actinobacillus pleuropneumoniae was established. Based on the ApxIV gene of Actinobacillus pleuropneumoniae serotype 1, a set of six primers were designed. The detection limit of the LAMP assay completed within 45 min at 63 ℃was 102 copies of the target se- quence, which was 10 times more sensitive than that of the PCR assay. High species-specificity of the LAMP method were confirmed by the assay of 18 pathogens such as A. pleuropneumoniae serotypes 1~10, Pasteurella multocida, Streptococcus suis and Mycoplasma hyopneumoniae. In addition, all the nasal swab samples from 8 experimental pigs infected by Actinobacillus pleuropneumoniae and 127 clinical cases were detected by LAMP assay, which deteleion rate was 100%, and the sensitivity is preferable to the PCR assay." @default.
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- W647613868 date "2009-01-01" @default.
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- W647613868 title "Detection of Actinobacillus pleuropneumoniae by Newly Developed Loop-mediated Isothermal Amplification Method" @default.
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