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- W64810600 abstract "Although the proteolytic activity of the Cu(II) complex of cyclen (Cyc) is greatly enhanced upon attachment to a cross-linked polystyrene (PS), the Cu(II)Cyc-containing PS derivatives reported previously hydrolyzed only a very limited number of proteins. The PS-based artificial metalloproteases can overcome thermal, mechanical, and chemical instabilities of natural proteases, but the narrow substrate selectivity of the artificial metalloproteases limits their industrial application. In the present study, artificial metalloproteases exhibiting broad substrate selectivity were synthesized by attaching Cu(II)Cyc to a PS derivative using linkers with various structures in an attempt to facilitate the interaction of various protein substrates with the PS surface. The new artificial metalloproteases hydrolyzed all of the four protein substrates (albumin, myoglobin, <TEX>${gamma}$</TEx>-globulin, and lysozyme) examined, manifesting <TEX>$k_{cat}/K_m$</TEX> values of 28-1500 <TEX>$h_{-1}M_{-1}$</TEX> at 50 <TEX>$^{circ}C$</TEX>. The improvement in substrate selectivity is attributed to steric and/or polar interaction between the bound protein and the PS surface as well as the hydrophobicity of the microenvironment of the catalytic centers." @default.
- W64810600 created "2016-06-24" @default.
- W64810600 date "2004-12-20" @default.
- W64810600 modified "2023-10-01" @default.
- W64810600 title "Artificial Metalloproteases with Broad Substrate Selectivity Constructed on Polystyrene" @default.
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- W64810600 doi "https://doi.org/10.5012/bkcs.2004.25.12.1917" @default.
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