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- W64854763 abstract "Abstract A high pressure liquid chromatographic method was developed using high pressure gel permeation chromatography (HP-GPC) and high pressure reverse phase chromatography (RP-HPLC) for quantitation of retinyl palmitate and β-carotene. HP-GPC was used for fractionation of vitamin A active compounds from oil preliminary to quantitation on nonaqueous RP-HPLC. HP-GPC fractionation was completed on oil and margarine dissolved in methylene chloride by 2 elution passes through 2 μStyragel (100Å) columns connected in series with methylene chloride as the mobile phase. RPHPLC separation of retinyl palmitate and β-carotene was achieved on μBondapak C18 (10 μm), using methylene chloride-acetonitrile ( 3 0 + 7 0 ) . Based on 10 repetitive analyses, recoveries of added β-carotene and retinyl palmitate from vegetable oils were 98.6±2.9 and 95.2±2.6%, respectively. The coefficients of variation were 2.9% for β-carotene and 2.7% for retinyl palmitate. The determination of vitamin A activity in 7 margarine brands with label claims of 10% U.S.RDA/serving revealed that all but one of the margarines contained at least 94% of the label claim. Vitamin A activity in the margarines ranged from 90.6 to 110.8% of the label declaration." @default.
- W64854763 created "2016-06-24" @default.
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- W64854763 date "1979-03-01" @default.
- W64854763 modified "2023-09-23" @default.
- W64854763 title "Application of Gel Permeation Chromatography and Nonaqueous Reverse Phase Chromatography to High Pressure Liquid Chromatographic Determination of Retinyl Palmitate and β-Carotene in Oil and Margarine" @default.
- W64854763 doi "https://doi.org/10.1093/jaoac/62.2.283" @default.
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