Matches in SemOpenAlex for { <https://semopenalex.org/work/W65003847> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W65003847 abstract "The main objective of the project was to isolate and purify actinomycetes from primary lowland rainforest in Danum Valley that might serve as potential inhibitor against GSK-3p through screening system using transformed yeasts. A total of 12 soil samples have been collected. The isolation of actinomycetes was achieved through the use of humic acid-vitamin agar media. From the isolation media, a total of 31actinomycetes were successfully purified using Oatmeal media agar. Glycerol stocks were prepared from these purified actinomycetes for storage purpose using 20% glycerol. These 31 strains from the Oatmeal media agar were then grown in 10ml mannitol-peptone-glucose liquid media separately to obtain the secondary metabolites produced by them. After duration of 120 hours in shaking incubator, 10ml acetone wasadded into each liquid culture to lyse the cells and release the secondary metabolites. These acetone extracts were used for screening against GSK-3 using yeast strains H10,075 and H10, 079, with the former transformed with pKTlO-gsk3P and the latter transformed with YEP24-MCKl. The screening system is based on temperature sensitivity, in which these two transformed yeast strains are able suppress temperature sensitivity phenotype. The diameter of the halos formed around the discs at the 2 different temperatures (37°C and 25°C) determined the activity of the extract on the yeast. The formation of halos with bigger diameter at 37°C than that of 25°C indicated that the extract has potential inhibitory activity on the yeast. On the other hand, smaller halo at 37°C showed that the extract was toxic to the yeast. From a total of 115 extracts that were screened against GSK-3, only three (H11462, H11490 and H11647) of them showed potential inhibitory activity." @default.
- W65003847 created "2016-06-24" @default.
- W65003847 creator A5005955480 @default.
- W65003847 date "2005-01-01" @default.
- W65003847 modified "2023-09-26" @default.
- W65003847 title "Isolation and purification of actinomycetes that serve aspotential inhibitor of GSK3" @default.
- W65003847 hasPublicationYear "2005" @default.
- W65003847 type Work @default.
- W65003847 sameAs 65003847 @default.
- W65003847 citedByCount "0" @default.
- W65003847 crossrefType "journal-article" @default.
- W65003847 hasAuthorship W65003847A5005955480 @default.
- W65003847 hasConcept C100544194 @default.
- W65003847 hasConcept C145890019 @default.
- W65003847 hasConcept C185592680 @default.
- W65003847 hasConcept C2775941552 @default.
- W65003847 hasConcept C2778660310 @default.
- W65003847 hasConcept C2779222958 @default.
- W65003847 hasConcept C2780881558 @default.
- W65003847 hasConcept C2781213060 @default.
- W65003847 hasConcept C31903555 @default.
- W65003847 hasConcept C43617362 @default.
- W65003847 hasConcept C523546767 @default.
- W65003847 hasConcept C54355233 @default.
- W65003847 hasConcept C55493867 @default.
- W65003847 hasConcept C62643968 @default.
- W65003847 hasConcept C86803240 @default.
- W65003847 hasConcept C89423630 @default.
- W65003847 hasConceptScore W65003847C100544194 @default.
- W65003847 hasConceptScore W65003847C145890019 @default.
- W65003847 hasConceptScore W65003847C185592680 @default.
- W65003847 hasConceptScore W65003847C2775941552 @default.
- W65003847 hasConceptScore W65003847C2778660310 @default.
- W65003847 hasConceptScore W65003847C2779222958 @default.
- W65003847 hasConceptScore W65003847C2780881558 @default.
- W65003847 hasConceptScore W65003847C2781213060 @default.
- W65003847 hasConceptScore W65003847C31903555 @default.
- W65003847 hasConceptScore W65003847C43617362 @default.
- W65003847 hasConceptScore W65003847C523546767 @default.
- W65003847 hasConceptScore W65003847C54355233 @default.
- W65003847 hasConceptScore W65003847C55493867 @default.
- W65003847 hasConceptScore W65003847C62643968 @default.
- W65003847 hasConceptScore W65003847C86803240 @default.
- W65003847 hasConceptScore W65003847C89423630 @default.
- W65003847 hasLocation W650038471 @default.
- W65003847 hasOpenAccess W65003847 @default.
- W65003847 hasPrimaryLocation W650038471 @default.
- W65003847 hasRelatedWork W16535633 @default.
- W65003847 hasRelatedWork W1929963713 @default.
- W65003847 hasRelatedWork W2007951487 @default.
- W65003847 hasRelatedWork W2052439657 @default.
- W65003847 hasRelatedWork W2079151120 @default.
- W65003847 hasRelatedWork W2085280236 @default.
- W65003847 hasRelatedWork W2186944302 @default.
- W65003847 hasRelatedWork W2217754312 @default.
- W65003847 hasRelatedWork W2892058109 @default.
- W65003847 hasRelatedWork W2970058735 @default.
- W65003847 hasRelatedWork W3009404340 @default.
- W65003847 hasRelatedWork W3010468038 @default.
- W65003847 hasRelatedWork W3046763469 @default.
- W65003847 hasRelatedWork W3092007920 @default.
- W65003847 hasRelatedWork W3173531241 @default.
- W65003847 hasRelatedWork W2338094497 @default.
- W65003847 hasRelatedWork W2510640752 @default.
- W65003847 hasRelatedWork W2828451833 @default.
- W65003847 hasRelatedWork W2838091638 @default.
- W65003847 hasRelatedWork W2847077094 @default.
- W65003847 isParatext "false" @default.
- W65003847 isRetracted "false" @default.
- W65003847 magId "65003847" @default.
- W65003847 workType "article" @default.