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- W650539746 abstract "The present experiment was conducted to establish the methodology of identification,separation,purification and cultivation of buffalo alveolar macrophage(AM) and to study the biological function of buffalo macrophage.Buffalo AM was isolated from lung-lavaging with cold PBS,the isolated cells were characterized functionally and phenotypically.The buffalo anchorage cells were obtained in large quantities which showed typical morphological characteristics of Mφ as observed by optical microscope and electron microscope.The positive rates of acid phosphatase and non-specific esterase were 89.4±3.5 and 83.6±3.4%,respectively.The positive rate of typical functional membrane proteins such as MHCⅡ was found to be 96.6±1.3%.The phagocytic rates of ink and chicken red blood cells were 92.3± 0.6 and 23.6 ± 4.2%,respectively.Therefore,the lung-lavaging with cold PBS was an efficient in vitro culture method for obtaining buffalo macrophage." @default.
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- W650539746 date "2010-01-01" @default.
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- W650539746 title "Identification, isolation and cultivation of buffalo alveolar macrophage." @default.
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