FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W650640962> ?p ?o ?g. }

• W650640962 endingPage "3910" @default.
• W650640962 startingPage "3901" @default.
• W650640962 abstract "Thermoalkalophilic lipases are prone to aggregation from their dimer interface to which structural zinc is very closely located. Structural zinc sites have been shown to induce protein aggregation, but the interaction between zinc and aggregation tendency in thermoalkalophilic lipases remains elusive. Here we delineate the interplay between zinc and aggregation of the lipase from Bacillus thermocatenulatus (BTL2), which is taken to be a representative of thermoalkalophilic lipase. Results showed that zinc removal disrupted the BTL2 dimer, leading to monomer formation and reduced thermostability manifesting as a link between zinc and dimerization that leads to thermostability, while zinc addition induced aggregation. Biochemical and kinetic characterizations of zinc-induced aggregates showed that the aggregates obtained from the early and late stages of aggregation had differential characteristics. In the early stages, the aggregates were soluble and possessed native-like structures, while in the late stages, the aggregates became insoluble and showed fibrillar characteristics with binding affinities for Congo red and thioflavin T. The impact of temperature on zinc-induced aggregation was further investigated, and it was found that the native-like early aggregates could completely dissociate into functional lipase forms at high temperatures while dissociation of the late aggregates was limited. To this end, we report that the zinc-induced aggregation of BTL2 can be reversed by temperature switches and initiated by ordered aggregates in the early stages that gain fibrillar-like features over time. Insights revealed by this work contributes to the knowledge of aggregation mechanisms that exist in thermophilic proteins, reflecting the potential use of metal addition and/or removal to fine-tune aggregation tendency." @default.
• W650640962 created "2016-06-24" @default.
• W650640962 creator A5015494378 @default.
• W650640962 creator A5039717404 @default.
• W650640962 date "2015-06-22" @default.
• W650640962 modified "2023-09-24" @default.
• W650640962 title "Zinc Modulates Self-Assembly of <i>Bacillus thermocatenulatus</i> Lipase" @default.
• W650640962 cites W1545803103 @default.
• W650640962 cites W1565076702 @default.
• W650640962 cites W1588296570 @default.
• W650640962 cites W1963994022 @default.
• W650640962 cites W1964048230 @default.
• W650640962 cites W1967222748 @default.
• W650640962 cites W1967549143 @default.
• W650640962 cites W1968041782 @default.
• W650640962 cites W1984113719 @default.
• W650640962 cites W1985339128 @default.
• W650640962 cites W1987075026 @default.
• W650640962 cites W1990835251 @default.
• W650640962 cites W1993914324 @default.
• W650640962 cites W1995102275 @default.
• W650640962 cites W1997566863 @default.
• W650640962 cites W1997827822 @default.
• W650640962 cites W2001090783 @default.
• W650640962 cites W2002559713 @default.
• W650640962 cites W2005123706 @default.
• W650640962 cites W2012618268 @default.
• W650640962 cites W2021064682 @default.
• W650640962 cites W2028046347 @default.
• W650640962 cites W2029667189 @default.
• W650640962 cites W2034027211 @default.
• W650640962 cites W2037158866 @default.
• W650640962 cites W2041499235 @default.
• W650640962 cites W2042885374 @default.
• W650640962 cites W2044646870 @default.
• W650640962 cites W2051023003 @default.
• W650640962 cites W2051564837 @default.
• W650640962 cites W2057709942 @default.
• W650640962 cites W2060442204 @default.
• W650640962 cites W2060495639 @default.
• W650640962 cites W2063281989 @default.
• W650640962 cites W2066767743 @default.
• W650640962 cites W2077057721 @default.
• W650640962 cites W2081186248 @default.
• W650640962 cites W2083334033 @default.
• W650640962 cites W2084815032 @default.
• W650640962 cites W2085408130 @default.
• W650640962 cites W2091654711 @default.
• W650640962 cites W2098079657 @default.
• W650640962 cites W2101389149 @default.
• W650640962 cites W2105740620 @default.
• W650640962 cites W2110193116 @default.
• W650640962 cites W2117661407 @default.
• W650640962 cites W2118385915 @default.
• W650640962 cites W2122301604 @default.
• W650640962 cites W2140245090 @default.
• W650640962 cites W2146554687 @default.
• W650640962 cites W2161819815 @default.
• W650640962 cites W2163210644 @default.
• W650640962 cites W2166788054 @default.
• W650640962 cites W2414307244 @default.
• W650640962 cites W2496025240 @default.
• W650640962 cites W66561613 @default.
• W650640962 doi "https://doi.org/10.1021/acs.biochem.5b00200" @default.
• W650640962 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26057387" @default.
• W650640962 hasPublicationYear "2015" @default.
• W650640962 type Work @default.
• W650640962 sameAs 650640962 @default.
• W650640962 citedByCount "13" @default.
• W650640962 countsByYear W6506409622015 @default.
• W650640962 countsByYear W6506409622016 @default.
• W650640962 countsByYear W6506409622018 @default.
• W650640962 countsByYear W6506409622019 @default.
• W650640962 countsByYear W6506409622020 @default.
• W650640962 countsByYear W6506409622022 @default.
• W650640962 countsByYear W6506409622023 @default.
• W650640962 crossrefType "journal-article" @default.
• W650640962 hasAuthorship W650640962A5015494378 @default.
• W650640962 hasAuthorship W650640962A5039717404 @default.
• W650640962 hasConcept C102931765 @default.
• W650640962 hasConcept C12554922 @default.
• W650640962 hasConcept C136238340 @default.
• W650640962 hasConcept C166940927 @default.
• W650640962 hasConcept C178790620 @default.
• W650640962 hasConcept C181199279 @default.
• W650640962 hasConcept C185592680 @default.
• W650640962 hasConcept C2779546866 @default.
• W650640962 hasConcept C2779697368 @default.
• W650640962 hasConcept C2781216036 @default.
• W650640962 hasConcept C521977710 @default.
• W650640962 hasConcept C535196362 @default.
• W650640962 hasConcept C55493867 @default.
• W650640962 hasConcept C86803240 @default.
• W650640962 hasConceptScore W650640962C102931765 @default.
• W650640962 hasConceptScore W650640962C12554922 @default.
• W650640962 hasConceptScore W650640962C136238340 @default.
• W650640962 hasConceptScore W650640962C166940927 @default.
• W650640962 hasConceptScore W650640962C178790620 @default.

• next