Matches in SemOpenAlex for { <https://semopenalex.org/work/W653530586> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W653530586 abstract "Nitronate monooxygenase (E.C. 1.13.12.16; NMO) is a detoxifying enzyme that oxidizes its substrate using a non-covalently linked cofactor, flavin mononucleotide (FMN). Substrate oxidation occurs through the formation of an anionic flavosemiquinone with oxygen as the final electron acceptor [1]. One oxygen atom is incorporated into the final product allowing for the classification of the enzyme as a monooxygenase. Propionate-3-nitronate (P3N) has been recently published to be the physiological substrate of NMO. P3N is the conjugate base of 3nitroproprionic acid and is highly toxic to succinate dehydrogenase and fumarase in the citric acid cycle. Inactivation of these essential enzymes by P3N leads to neurological disorders or death [2]. In this study, nitronate monooxygenase from Williopsis saturnus was expressed in Escherichia coli and purified to high levels through ammonium sulfate fractionation and two chromatographic steps. This procedure yielded an improved preparation with respect to that previously published in both specific activity and amount of enzyme. The purified enzyme was used to set up crystallization trials in order to obtain suitable crystals for X-ray crystallography. To set up protein crystals, nitronate monooxygenase was first concentrated through ultrafiltration and subjected to size exclusion column chromatography to remove free flavin from the enzyme solution. This study reports the current state of the project with an aim for the elucidation of the three dimensional structure of this important enzyme." @default.
- W653530586 created "2016-06-24" @default.
- W653530586 creator A5033370880 @default.
- W653530586 date "2013-01-01" @default.
- W653530586 modified "2023-09-26" @default.
- W653530586 title "Purification and Crystallization of Nitronate Monooxygenase: a Detoxifying Enzyme from Williopsus saturnus" @default.
- W653530586 hasPublicationYear "2013" @default.
- W653530586 type Work @default.
- W653530586 sameAs 653530586 @default.
- W653530586 citedByCount "0" @default.
- W653530586 crossrefType "journal-article" @default.
- W653530586 hasAuthorship W653530586A5033370880 @default.
- W653530586 hasConcept C13002179 @default.
- W653530586 hasConcept C178790620 @default.
- W653530586 hasConcept C181199279 @default.
- W653530586 hasConcept C185592680 @default.
- W653530586 hasConcept C18903297 @default.
- W653530586 hasConcept C2776343937 @default.
- W653530586 hasConcept C2777289219 @default.
- W653530586 hasConcept C2777427158 @default.
- W653530586 hasConcept C2780263894 @default.
- W653530586 hasConcept C2780639532 @default.
- W653530586 hasConcept C33093398 @default.
- W653530586 hasConcept C526171541 @default.
- W653530586 hasConcept C71240020 @default.
- W653530586 hasConcept C71995715 @default.
- W653530586 hasConcept C86803240 @default.
- W653530586 hasConceptScore W653530586C13002179 @default.
- W653530586 hasConceptScore W653530586C178790620 @default.
- W653530586 hasConceptScore W653530586C181199279 @default.
- W653530586 hasConceptScore W653530586C185592680 @default.
- W653530586 hasConceptScore W653530586C18903297 @default.
- W653530586 hasConceptScore W653530586C2776343937 @default.
- W653530586 hasConceptScore W653530586C2777289219 @default.
- W653530586 hasConceptScore W653530586C2777427158 @default.
- W653530586 hasConceptScore W653530586C2780263894 @default.
- W653530586 hasConceptScore W653530586C2780639532 @default.
- W653530586 hasConceptScore W653530586C33093398 @default.
- W653530586 hasConceptScore W653530586C526171541 @default.
- W653530586 hasConceptScore W653530586C71240020 @default.
- W653530586 hasConceptScore W653530586C71995715 @default.
- W653530586 hasConceptScore W653530586C86803240 @default.
- W653530586 hasLocation W6535305861 @default.
- W653530586 hasOpenAccess W653530586 @default.
- W653530586 hasPrimaryLocation W6535305861 @default.
- W653530586 hasRelatedWork W1473874081 @default.
- W653530586 hasRelatedWork W1503496924 @default.
- W653530586 hasRelatedWork W1548770541 @default.
- W653530586 hasRelatedWork W1917272347 @default.
- W653530586 hasRelatedWork W1966173148 @default.
- W653530586 hasRelatedWork W2003201051 @default.
- W653530586 hasRelatedWork W2029370037 @default.
- W653530586 hasRelatedWork W2040924301 @default.
- W653530586 hasRelatedWork W2050205551 @default.
- W653530586 hasRelatedWork W2073593925 @default.
- W653530586 hasRelatedWork W2079108910 @default.
- W653530586 hasRelatedWork W2084633195 @default.
- W653530586 hasRelatedWork W2092978726 @default.
- W653530586 hasRelatedWork W2154110018 @default.
- W653530586 hasRelatedWork W985668934 @default.
- W653530586 hasRelatedWork W1534961088 @default.
- W653530586 hasRelatedWork W2001809808 @default.
- W653530586 hasRelatedWork W2755348422 @default.
- W653530586 hasRelatedWork W2769279048 @default.
- W653530586 hasRelatedWork W2923740948 @default.
- W653530586 isParatext "false" @default.
- W653530586 isRetracted "false" @default.
- W653530586 magId "653530586" @default.
- W653530586 workType "article" @default.