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- W654350857 abstract "Tropical freshwater prawns are of considerable interest because of the importanceof this commodity in international trade. They are one of the highest valuedproducts in international fisheries trade and as such have good long term potential.Studies on the bacteriological and biochemical changes 'of pond water and culturedfreshwater prawns were carried out to evaluate the relationship between waterquality and microbiological changes during storage. Prawns were taken from threesampling sites viz: Site 1- Kg. Jumbang, Negri Sembilan; Site 2- Kg. Cangkat Tin,Perak and Site 3- Kg. Cenderiang, Perak. They were then stored at 3 differentstorage conditions: 1) 20 d at ambient (280 ± 2°C); 2) 10 d at 100 ± 2°C and 3) 16 dat iced storage (4° ± 2°C). Microbiological analysis was performed for totalmesophilic and psychrophilic aerobic COWlts, proteolytic bacterial COWlts, histamineproducing bacteria, cadaverine producing bacteria and putrescine producingbacteria in the prawns and pond water for the three sites using appropriate bacteriological agar media. Biochemical analysis was carried out for pH profilesusing pH meter, freshness in terms of K-values from the test paper strips, aminoacids profiles using the amino acid analyser, total volatile bases (TVB) and totalvolatile acids (TVA) from the distillation methods and biogenic amines viz;histamine, cadaverine and putrescine using the high performance liquidchromatography (HPLC) method. Results obtained showed that themicrobiological quality of freshwater prawns was related to the micro flora of pondwater where they were grown. The initial counts indicated the values were in therange of log 4+ CFU/g for all samples. Total mesophilic and psychrophilic countsof the head regions were higher than that of the body regions for all prawn samplesand types of growth media tested. All samples showed an increase in counts withtime and temperature of storage up to log 7+ CFU/g for mesophilic counts after 12hat ambient, 6 d at lO˚C and 12 d at iced storage. Site 2 samples had relativelyhigher counts as compared to that of the other two sites which correlated well withthe levels determined in their pond water. Similar trends were observed forpsychrophilic counts but at lower values for different types of media studied.Biochemical results showed similar trends of increment in values and were closelyrelated to the microbiological data until spoilage set in.Effect of preservatives on quality changes, biogenic amines production andshelf life of prawns during iced storage were investigated by sensory,microbiological, physical and chemical analyses. Sensory results indicated that thewhole prawns had a shelf life of about 20 d when treated with 2% sucrose, boricacid, lactic acid, sodium chloride, and sodium metabisulfite except for control which had only 15 d shelf life. Boric acid, lactic acid and sodium metabisulfitemanaged to inhibit psychrophilic bacteria and biogenic amines formation in prawnswhile maintaining the mesophilic counts at lower levels during iced storage.Different analytical methods for the determination of histamine in prawnswere evaluated. Use of enzymatic methods in the form of oxygen electrode andspectrophotometer offered excellent alternative methods for determination ofhistamine in freshwater prawns. The experimental results showed there was anincrease of histamine measured by the oxygen electrode and very good correlationof above 0.98 with the HPLC analyser for all samples. Recovery percentages ofabout 100% for values of histamine between 0-200 µg/g was observed. Similarly,use of spectrophotometer with coupled diamine oxidase and horseradish peroxidasein the system allowed a semi quantitative estimation of histamine in prawns.Correlation coefficients of r2= 0.99 were observed when compared to that of theHPLC method. Percentages of recovery of histamine in augmented samples werealso about 100% for concentration of histamine between 0-200 µg/g." @default.
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- W654350857 date "2001-03-01" @default.
- W654350857 modified "2023-09-27" @default.
- W654350857 title "Microbiological and Biochemical Changes of Freshwater Prawns (Macrobrachium Rosenbergii) During Storage" @default.
- W654350857 hasPublicationYear "2001" @default.
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