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• W655694570 abstract "Environmental isolates belonging to the genus Acidovorax play a crucial role in degrading a wide range of pollutants. Studies on Acidovorax are currently limited for many species due to the lack of genetic tools. Here, we described the use of the replicon from a small, cryptic plasmid indigenous to Acidovorx temperans strain CB2, to generate stably maintained shuttle vectors. In addition, we have developed a scarless gene knockout technique, as well as establishing green fluorescent protein (GFP) reporter and complementation systems. Taken collectively, these tools will improve genetic manipulations in the genus Acidovorax." @default.
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• W655694570 date "2015-08-01" @default.
• W655694570 modified "2023-09-26" @default.
• W655694570 title "The genetic toolbox for Acidovorax temperans" @default.
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• W655694570 doi "https://doi.org/10.1016/j.mimet.2015.06.006" @default.
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