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- W655707890 abstract "Label-free monitoring of biomolecular reactions in real-time is of great interest since it can provide direct results concerning binding kinetics and equilibrium constants. In this report, a method based on White Light Reflectance Spectroscopy (WLRS) is presented that is capable for real-time monitoring of biomolecular reactions taking place on a solid surface. The optical setup consists of a VIS–NIR light source connected through a bifurcated optical fiber to a PC-driven spectrophotometer. The outer part of the optical fibre guides the light vertically onto the surface where the biomolecular reactions occur. The reflected light is collected from the central part of the optical fibre and is directed to the spectrophotometer. The reactions take place on the top of a polymer covered silicon dioxide surface. A microfluidic module in combination with a micropump is used to supply the reagents in an adjustable rate. The reflectance signal is recorded in real-time. The biomolecular interactions are monitored as shifts of the wavelength where constructive interference is observed. These wavelength shifts correspond to biomolecular film thickness changes during either the adsorption of biomolecules onto the substrate or during biomolecule’s reaction with the immobilized counterpart molecules. The proposed methodology has been applied for real-time and label-free monitoring of streptavidin binding to surface-immobilised biotinylated protein as well as of mouse IgG onto immobilized anti-mouse IgG antibody. Mouse IgG at concentrations less than 150 pM were detected in short reaction times (10 min). The proposed reflectance spectroscopy sensor provides a simple, fast, low cost approach for label-free monitoring of biomolecular interactions thus making the developed sensor suitable for various analytical applications." @default.
- W655707890 created "2016-06-24" @default.
- W655707890 creator A5034006526 @default.
- W655707890 date "2009-05-29" @default.
- W655707890 modified "2023-09-27" @default.
- W655707890 title "Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις" @default.
- W655707890 hasPublicationYear "2009" @default.
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