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- W655988574 abstract "This study was conducted to determine the occurrence and prevalence of methicillin resistant Staphylococcus aureus (MRSA) in finishing pigs on-farm, at lairage and assess the likelihood of carriage at slaughter and retail levels. A crosssectional study targeting ten cohorts of commercial swine farms was conducted for carriage of MRSA. Paired nasal and peri-anal swab samples (n=24/farm) were collected from market age pigs on-farm and the same batch of pigs were followed and sampled at the lairage before slaughter and carcass swabs at post evisceration stage before chilling. Pork samples from the same batch of pigs were collected at retail market. We assessed phenotypic and genotypic relatedness from the various sources. Conventional cultural methods using oxacillin resistance screening agar was used. Antimicrobial resistance was tested to a panel of 21 antimicrobials. PCR was used to detect the presence of species-specific gene (nuc) and methicillin resistance marker gene (mecA). The genotypic relatedness of isolates was determined using the Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). One or more MRSA positive pigs were detected in five of the ten herds (50%). The prevalence of MRSA in pigs was higher at lairage and ranged from 0% to 54.2% per farm compared to that same batch of pigs on-farm (0% to 12.5%). The proportion of MRSA positive isolates recovered from nasal swab samples was relatively higher (4.8%) compared to peri-anal samples (2.7%). We detected MRSA in 1.6% (4/240) of the carcass swab and 3.7% (5/135%) of the retail pork samples. Genotypically similar isolates were detected from farm to the retail chain based on PFGE. Using MLST, ST398 was detected from farm, lairage and retail pork. In addition ST5, ST9, ST39 and ST72 were detected at different points of sampling." @default.
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- W655988574 date "2011-01-01" @default.
- W655988574 modified "2023-10-14" @default.
- W655988574 title "Methicillin Resistant Staphylococcus aureus (MRSA) in market age pigs on-farm, at slaughter and retail pork" @default.
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- W655988574 doi "https://doi.org/10.31274/safepork-180809-588" @default.
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