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- W656579811 abstract "11 CHAPTER 1-INTRODUCTION 13 EPIDEMIOLOGY OF ASTHMA 13 PATHOGENESIS OF ASTHMA 13 BIOMARKERS DEFINITIONS 16 VALUE OF BIOMARKERS 16 BEYOND BIOMARKERS THEMSELVES 18 IN VIVO AND IN VITRO STUDIES 18 PROTEOMICS 19 BIOMARKERS AND ASTHMA 23 HYPOTHESES 24 CHAPTER 2MATERIALS AND METHODS 26 BIRTH COHORT SUBGROUPS AND SAMPLES 26 ASTHMA BIOMARKER DISCOVERY (PARENT PROJECT) 27 ANTIBODY ARRAY STUDIES 27 ELISA QUANTITATION OF CANDIDATE PROTEINS 29 IN VIVO STUDIES OF EPO’S BIOLOGICAL EFFECTS 31 Bronchoconstriction Studies in Unsensitized Mouse Model 31 Airway Hyperresponsiveness Mouse Model 34 Specimen Collection 36 ELISA Quantitation of EPO 36 IN VITRO STUDIES OF EPO’S BIOLOGICAL EFFECTS 37 Peripheral Blood Mononuclear Cell (PBMC) Isolation 37 PBMC Cell Count 37 Cell Pretreatment 38 Mitogen and LPS Stimulation 38 ELISA Quantitation of IL-2 and IL-6 39 STATISTICAL ANALYSIS 39 CHAPTER 3RESULTS: ANTIBODY ARRAY STUDIES 42 (THE FOLLOWING LIST OF QUESTIONS WERE ADDRESSED IN THIS CHAPTER) DOES ASTHMA HAVE IDENTIFIABLE COMPONENTS IN THE SYSTEMIC CIRCULATION? 42 Identification of protein candidate biomarkers in plasma for childhood asthma through antibody arrays 42" @default.
- W656579811 created "2016-06-24" @default.
- W656579811 creator A5008871494 @default.
- W656579811 date "2014-01-01" @default.
- W656579811 modified "2023-10-12" @default.
- W656579811 title "Discovery And Validation Of Early Life Plasma Protein Biomarkers For Childhood Asthma" @default.
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