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• W656899162 abstract "Mesenchymal stem cells (MSCs) are uniquely capable of crossing germinativelayers borders (these cell populations are able to differentiate towardsectoderm-, mesoderm- and endoderm- derived lineages) and are viewed aspromising cells for regenerative medicine approaches in several diseases.Some undoubtedly limiting factors for the clinical use of MSCs, i.e. for the repairof bone defects, are related to many different problems, that only partially todaycan be overcome through ex-vivo expansion and cells modification strategies.Considering MSCs sources, the use of fetal annexes-derived MSCs, such asMSCs from Whartonʼs jelly of umbilical cord (WJMSCs), or the recruitment ofMSCs from “stem cell niches” in adult discard tissues, like periodontal ligament(PDL) of extracted teeth (PDLMSCs), could be promising in compensating limitsof MSCs traditional sources, i.e. Bone Marrow, like small cells number, highharvesting technique morbility, difficult cells commitment due to earlysenescence. Uniforming and investigating best cells culture conditions with in vitro differentexperimental strategies is useful in our job to understand and controldifferentiation mechanisms and finally to influence the yield and proliferationrate of these MSCs populations, together with their osteogenic potential.The aim of our study is briefly sumarized:- To isolate and culture MSCs cells from human Umbilical Cord Whartonʼs Jellyand Periodontal Ligament;- To compare characteristics between all samples recruited and to link them toclinical aspects of tissue donors;- To characterise both MSCs population in regard to their proliferation anddifferentiation potential;- To investigate their functional characteristics before and after alginatemicrobeads encapsulation;- To investigate effects of three-dimensional systems and microgravity onMSCs before and after differentiation.WJMSCs and PDLSCs were analyzed for the expression of MSC markers, andthen committed to osteogenic differentiation. Before and after differentiation,alkaline phosphatase (ALP) activity, the expression level of a specific osteoblasttranscription factor (Runx2), and mineralization status were evaluated. Theperformance of WJMSCs and PDLSCs was then compared in 3-D culturesystems (alginate beads and bioreactor system) in terms of viability,proliferation, secretive profile, expression of markers and effectiveness ofphenotype modulation.Characterization of MSCs population was succesfull for all samplesinvestigated, and for largest samples cohort (WJMSCs) it was possible tocorrelate cells biochemical parameters to clinical donors features. Thesefindings may help during selection of best donors to combine them withscaffolds and biomaterials to promote tissue regeneration.For both MSCs populations, we demonstrated that cells can live and grow in 3-D systems. All MSCs samples analyzed showed a substantial osteogenicpotential, before and after encapsulation in alginate microbeads.Modulation of cells culture conditions, with the use of nanotechnologiesstrategies or the use of bioreactors, like Rotary Cell Culture SystemTM(RCCS-4TM bioreactor, SyntheconTM, Inc., Houston, TX, U.S.A.) with HighAspect Ratio Vessel (HARVTM) has been shown to be a useful and promisingapproach to investigate characteristics and environmental effects of cells/biomaterials combinations, in order to predict their effect and potential forregenerative medicine strategies." @default.
• W656899162 created "2016-06-24" @default.
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• W656899162 date "2011-02-22" @default.
• W656899162 modified "2023-09-24" @default.
• W656899162 title "Mesenchymal stem cells from Wharton's Jelly and periodontal ligament:reliable not controversial sources for osteogenic differentiation andregenerative medicine." @default.
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