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- W657614802 abstract "Although primordial germ cells (PGCs) have been used in the production of germline chimera, efficiency has not been satisfactory. The present study was conducted to improve efficiency of germline chimera production using the cultured gonadal PGCs (gPGCs). Germline chimeric chickens were produced by transfer of cultured gonadal primordial germ cells from Korean Ogol Chicken (KOC) to White Leghorn (WL). Gonadal PGCs were isolated from KOC embryonic gonads at stage 27 (5.5-day-old) and cultured in vitro for 10 days. Approximately 200 gPGCs were injected into the bloodstream through the dorsal aorta of stage 12-13 (2-day-old) recipient embryos from which blood had been withdrawn via the dorsal aorta prior to the injection. Recipient embryos were incubated until hatching. Germline chimerism of the chickens reaching maturity was examined by mating them with Korean Ogol Chicken. Donor-derived offspring were identified as germline chimeric chickens based on their feather color. The frequency of germline transmission of donor PGCs ranged 1.9-60.7%. There was no difference between both sexes. Therefore, it can be concluded that efficiency of germline chimerism can be improved via using cultured gPGCs." @default.
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- W657614802 date "2000-11-01" @default.
- W657614802 modified "2023-09-24" @default.
- W657614802 title "High Transmission Rate of Germline Chimerism Using Cultured Primordial Germ Cells in Chickens." @default.
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