Matches in SemOpenAlex for { <https://semopenalex.org/work/W66610077> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W66610077 endingPage "395" @default.
- W66610077 startingPage "393" @default.
- W66610077 abstract "Cytodex 3 microcarriers were used for the production of an active nerve growth promoting factor from chicken heart cells. Such cells release into their culture medium a substance which stimulates the growth of nerve fibres from explanted ciliary, spinal and sympathetic neurons. In addition, culture in serum-free medium reduces the presence of contaminating proteins and facilitates biochemical analysis of this factor. A mixture of DME/FIO was supplemented with either 10% FCS, or a low molecular weight fraction of FCS, or different hormones and growth factors. Cells cultured in media supplemented with insulin, transferrin, human serum albumin and fibronectin in combination with 10% FCS-dialysate or a mixture of FGF, EGF, dexamethasone, calmodulin and thrombin progressed through the cell cycle with kinetics similar to those obtained with 10% FCS. Maximum amount of cells in (S+G2) phase was obtained after 18–20 hrs. The cells proliferated on Cytodex 3 at a rate similar to cells grown on cell culture plastic. The results confirm the potential of microcarrier cell culture in serum-free media for scaling up the high yield production of a specific cell product.KeywordsHuman Serum AlbuminChicken EmbryoSympathetic NeuronHeart CellHigh Yield ProductionThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves." @default.
- W66610077 created "2016-06-24" @default.
- W66610077 creator A5024046336 @default.
- W66610077 creator A5057788244 @default.
- W66610077 creator A5068005996 @default.
- W66610077 creator A5075168316 @default.
- W66610077 date "1983-01-01" @default.
- W66610077 modified "2023-09-27" @default.
- W66610077 title "The Use of Cytodex 3 Microcarriers and Serum-Free Media for the Production of a Nerve Growth Promoter from Chicken Heart Cells" @default.
- W66610077 cites W1997091660 @default.
- W66610077 cites W2022360237 @default.
- W66610077 cites W2023688577 @default.
- W66610077 cites W2033474619 @default.
- W66610077 cites W2049412913 @default.
- W66610077 doi "https://doi.org/10.1007/978-3-642-69290-1_58" @default.
- W66610077 hasPublicationYear "1983" @default.
- W66610077 type Work @default.
- W66610077 sameAs 66610077 @default.
- W66610077 citedByCount "1" @default.
- W66610077 crossrefType "book-chapter" @default.
- W66610077 hasAuthorship W66610077A5024046336 @default.
- W66610077 hasAuthorship W66610077A5057788244 @default.
- W66610077 hasAuthorship W66610077A5068005996 @default.
- W66610077 hasAuthorship W66610077A5075168316 @default.
- W66610077 hasConcept C126322002 @default.
- W66610077 hasConcept C134018914 @default.
- W66610077 hasConcept C1491633281 @default.
- W66610077 hasConcept C153911025 @default.
- W66610077 hasConcept C170493617 @default.
- W66610077 hasConcept C176999471 @default.
- W66610077 hasConcept C185592680 @default.
- W66610077 hasConcept C202751555 @default.
- W66610077 hasConcept C2775960820 @default.
- W66610077 hasConcept C2778423431 @default.
- W66610077 hasConcept C2779404806 @default.
- W66610077 hasConcept C54355233 @default.
- W66610077 hasConcept C55493867 @default.
- W66610077 hasConcept C71924100 @default.
- W66610077 hasConcept C81885089 @default.
- W66610077 hasConcept C86803240 @default.
- W66610077 hasConcept C95444343 @default.
- W66610077 hasConcept C98648569 @default.
- W66610077 hasConceptScore W66610077C126322002 @default.
- W66610077 hasConceptScore W66610077C134018914 @default.
- W66610077 hasConceptScore W66610077C1491633281 @default.
- W66610077 hasConceptScore W66610077C153911025 @default.
- W66610077 hasConceptScore W66610077C170493617 @default.
- W66610077 hasConceptScore W66610077C176999471 @default.
- W66610077 hasConceptScore W66610077C185592680 @default.
- W66610077 hasConceptScore W66610077C202751555 @default.
- W66610077 hasConceptScore W66610077C2775960820 @default.
- W66610077 hasConceptScore W66610077C2778423431 @default.
- W66610077 hasConceptScore W66610077C2779404806 @default.
- W66610077 hasConceptScore W66610077C54355233 @default.
- W66610077 hasConceptScore W66610077C55493867 @default.
- W66610077 hasConceptScore W66610077C71924100 @default.
- W66610077 hasConceptScore W66610077C81885089 @default.
- W66610077 hasConceptScore W66610077C86803240 @default.
- W66610077 hasConceptScore W66610077C95444343 @default.
- W66610077 hasConceptScore W66610077C98648569 @default.
- W66610077 hasLocation W666100771 @default.
- W66610077 hasOpenAccess W66610077 @default.
- W66610077 hasPrimaryLocation W666100771 @default.
- W66610077 hasRelatedWork W126289829 @default.
- W66610077 hasRelatedWork W139077832 @default.
- W66610077 hasRelatedWork W1552993337 @default.
- W66610077 hasRelatedWork W1991713271 @default.
- W66610077 hasRelatedWork W1993750220 @default.
- W66610077 hasRelatedWork W1993859845 @default.
- W66610077 hasRelatedWork W1998358648 @default.
- W66610077 hasRelatedWork W2002005202 @default.
- W66610077 hasRelatedWork W2010935847 @default.
- W66610077 hasRelatedWork W2037330055 @default.
- W66610077 hasRelatedWork W2046386827 @default.
- W66610077 hasRelatedWork W2076632398 @default.
- W66610077 hasRelatedWork W2089459633 @default.
- W66610077 hasRelatedWork W2094057505 @default.
- W66610077 hasRelatedWork W2101566388 @default.
- W66610077 hasRelatedWork W2103687903 @default.
- W66610077 hasRelatedWork W2119802297 @default.
- W66610077 hasRelatedWork W2132409870 @default.
- W66610077 hasRelatedWork W2402934754 @default.
- W66610077 hasRelatedWork W2474677046 @default.
- W66610077 isParatext "false" @default.
- W66610077 isRetracted "false" @default.
- W66610077 magId "66610077" @default.
- W66610077 workType "book-chapter" @default.