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- W66707586 abstract "521 Objectives To date, with advancement of application of mesenchymal stem cells (MSCs) in biomedicine, in vivo tracking of MSCs in noninvasive, sensitive, quantitative, tomographic, and cell-nonhazardous ways is highly desired. Our study is to explore the advantages and disadvantages of tracking MSCs by uPET imaging using ex vivo 64Cu - pyruvaldehyde-bis (N4-methylthiosemicarbazone) (PTSM) cell labeling. Methods Murine bone marrow derived-MSCs were isolated and labeled with prepared 64Cu -PTSM. In vivo uPET imaging was conducted after injection of MSCs into Bab/c mice. Biodistribution of labeled MSCs at early and late time point were measured. Also, cell uptake and efflux assay were conducted. Finally, the impact of 64Cu -PTSM labeling on MSCs growth, differentiation were evaluated by cell counting and staining. Results 64Cu -PTSM labelling efficiency of MSCs was around 75%. Labeling has no impact on MSCs viability. In vivo uPET imaging of 64Cu -PTSM labeled MSCs demonstrated quick lung accumulation at early time point (4 h) after tail vein injection of MSCs and showed decreased lung signal with time. Cell uptake of 64Cu -PTSM by MSCs already reached plateau as early as 1 h (94.3%). Cell retention of 64Cu -PTSM decreased over time and the value were 94.4%, 82.7%, 66.2%, 19.2% and 18.7% respectively at 0.5, 1, 2, 4, 20, 24 hr time point. Biodistribution result showed that lung uptake of 64Cu-PTSM was 70.7% at 4 h time point, which indicated that MSCs could be quantitatively assessed at early time point by uPET imaging based on uptake and efflux assay results, however, late time point uPET imaging cannot reflect real distribution of labeled MSCs due to quick efflux of 64Cu -PTSM . In addition, radiation of 64Cu -PTSM had no significant impact on MSCs growth and differentiation measured by cell counting and staining. Conclusions This is the first study to investigate the feasibility of tracking MSCs by uPET imaging using ex vivo 64Cu -PTSM cell labeling. This MSCs labeling strategy has potential for certain future preclinical and clinical applications" @default.
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- W66707586 date "2011-05-01" @default.
- W66707586 modified "2023-09-24" @default.
- W66707586 title "In vivo PET imaging to track mesenchymal stem cells labelled with copper-64-pyruvaldehyde-bis (N4-methylthiosemicarbazone)" @default.
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