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- W66793339 abstract "The genus Paphiopedilum is endangered species protected under Convention on International Trade in Endangered Species (CITES). However, the discrimination between the species is difficult due to their similar morphological characters. Therefore, RAPD assay were applied to detect polymorphisms between the three species: P. rothschildianum, P. dayanum and P. lowii. Diagnostic fragments identified for discrimination for each species were converted into SCAR markers by cloning and sequencing the diagnostic fragments and designing SCAR primer pairs to amplify the diagnostic fragments. The reproducibility of these markers was analysed by performing diagnostic PCRs. In this study, PCR amplification with RAPD primers was successful indetecting polymorphisms between species. Three diagnostic fragments were converted into SCAR markers and all primer pairs amplified single distinct bands whose sizes werethe same as expected fragments. One of the SCAR primers amplified the expected product size in P. dayanum and in one of the P. rothschildianum samples. When digested with restriction enzyme DpnII found polymorph isms in fragment length between the two samples. In the other two cases, the products of the expected fragments were amplified in P. lowii and all samples of P. rothschildianum and subsequent RE digestion failed to detect polymorph isms between the species. The overall findings had shown discovery of two SCAR markers to identify P. rothschildianum and P. lowii apart from P. dayanum and one SCAR marker to identify P. dayanum and its possible hybrids. Hence, more studies and screenings need to be done in order to develop species-specific markers for the Paphiopedilum species." @default.
- W66793339 created "2016-06-24" @default.
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- W66793339 date "2007-01-01" @default.
- W66793339 modified "2023-09-27" @default.
- W66793339 title "Conversion of RAPD markers to co-dominant based Sequence Characterized Amplified Region (SCAR) Markers in three paphiopedilum species" @default.
- W66793339 hasPublicationYear "2007" @default.
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