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- W67101313 abstract "Human peripheral blood leucocytes respond in vitro to a variety of stimuli by undergoing a reaction that includes the enlargement of the lymphocytes, active synthesis of RNA and protein, incorporation of thymidine, and eventual division. The reaction is most commonly assayed by studying the incorporation. In an experiment, sixty to 300 ml of human peripheral blood is drawn into syringes that have been heparinized with 0.5 to 1 ml of heparin per 60 ml of blood drawn. Cultures in which the various factors are made are called stage I cultures. They contain an appropriate number of cells in medium with or without a stimulant. Control cultures consist of medium 199S: 10% made in parallel with the factor-producing culture started on the same day, made with the same plasma, placed in the same incubator, and harvested in the same way, except that no cells are added." @default.
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- W67101313 date "1971-01-01" @default.
- W67101313 modified "2023-10-14" @default.
- W67101313 title "LEUCOCYTE CULTURE METHODOLOGY" @default.
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- W67101313 doi "https://doi.org/10.1016/b978-0-12-107750-1.50042-x" @default.
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