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- W67205845 endingPage "216" @default.
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- W67205845 abstract "This chapter describes how the immobilization of bioreceptors on materials can be studied by various IR surface techniques for the elaboration of biosensors and recognition of target molecules. It emphasizes three of these techniques, which benefit from a surface-enhanced sensitivity, namely, attenuated total reflection infrared (ATR-IR) spectroscopy, surface enhanced infrared spectroscopy (SEIRAS), and eventually infrared reflection absorption spectroscopy (IRRAS). It also emphasizes the use of transition metal carbonyl and some other chemical probes in conjunction with IR spectroscopy for molecular sensing and biorecognition. For biosensing applications, analytical sensitivity is of critical importance. This chapter also explains and illustrates the use of metal carbonyl probes as well as some other good IR reporters. FTIR spectroscopy can be set up in various configurations, making the technique sensitive to very low amounts of molecules adsorbed on solid surfaces; it can be advantageously used to characterize biomolecular recognition phenomena. A large variety of high affinity association processes between two biological partners can be efficiently transduced. In some cases, the IR probes operate as molecular sensors, that is, their signal is affected by the association. While most of the published work involved transition metal carbonyl probes, the use of other chemical species with close spectral features (nitrile and azide groups) has been recently reported that shed light on various important biological processes." @default.
- W67205845 created "2016-06-24" @default.
- W67205845 creator A5043583635 @default.
- W67205845 creator A5051737470 @default.
- W67205845 creator A5067010131 @default.
- W67205845 date "2011-01-01" @default.
- W67205845 modified "2023-10-16" @default.
- W67205845 title "IR spectroscopy for biorecognition and molecular sensing" @default.
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