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• W67667036 abstract "SHANTI RATNAKOMALA. P055050031. Purification and characterization of protein inhibitor to RNA helicase enzyme of Japanese encephalitis virus from Streptomyces chartreusis 5-095. Under the direction of ANJA MERYANDINI and ANDI UTAMA. Japanese encephalitis virus (JEV), a member of flavivirus, is the most common cause of epidemic viral encephalitis. Several efforts have been performed to find a drug candidate for treatment of JEV infection, including finding of inhibitors of enzymes that essential for JEV replication. Streptomyces chartreusis 5-095 produce the inhibitor of JEV RNA helicase. In this study, an extracellular protein which has inhibition activity on JEV RNA helicase, particularly on its ATPase activity, was partially purified from supernatant of S. chartreusis 5-095 culture. The culture supernatant exhibited high inhibition activity against ATPase activity of JEV RNA helicase (approximately 43%) at 10 days of incubation. The protein inhibitor was purified by the procedures of precipitation with ammonium sulfate to 70% saturation, gel filtration (Sephadex G-50) using methanol water solution (40:60) as a mobile phase, Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC). The purity of protein inhibitor increased 9.75 fold than those of the crude protein. The specific activity after purification was 36.48 U/mg. Gradient polyacrilamide gel electrophoresis analysis showed that the relative molecular mass of the proteins were estimated to be 10 kDa and 14 kDa. The activity of purified protein inhibitor was optimum at pH 7.4, and maintained its stability over pH range from 4-9. The purified protein inhibitor relatively stable its activity at storage in the range of -20C to room temperature for 15 days of incubation." @default.
• W67667036 created "2016-06-24" @default.
• W67667036 creator A5027179435 @default.
• W67667036 date "2009-01-01" @default.
• W67667036 modified "2023-09-23" @default.
• W67667036 title "Purifikasi dan Karakterisasi Protein Inhibitor enzim RNA Helikase Japanese Encephalitis Virus dari Streptomyces chartreusis 5-095" @default.
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