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- W68306586 abstract "The extensive use of cell cultures for diverse research purposes is one of the truly great international growth industries. With the proliferation of cells comes a responsi bility for monitoring them for inter- and intraspecies characteristics. We use multiple genetic markers for cell identifi cation, i.e. species specific antigens, isozymic phenotypes, chromosomal complement, and HL-A haplotypes. The methodologies employed are briefly described, and various examples cited to show how these markers can be utilized for cell line monitoring. Data are summarized from 275 cultures sent to our laboratory for analysis during the past eighteen months. The data show that, overall, 35% of the cultures received were contaminated. The majority of cell cultures submitted were human cell lines. We found that 36% of these cultures were cross contaminated; 25% by cells of another species and 11% by another human cell line. This high incidence of inter- and intraspecies contamination under scores the importance of frequent monitoring of cell cultures." @default.
- W68306586 created "2016-06-24" @default.
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- W68306586 date "1984-01-01" @default.
- W68306586 modified "2023-09-25" @default.
- W68306586 title "Cell Characterization by Use of Multiple Genetic Markers" @default.
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- W68306586 doi "https://doi.org/10.1007/978-1-4615-9376-8_2" @default.
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