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• W68477947 abstract "In the post-genome stage, DNA information concerning individual people can be used for protection against or treatment of diseases. To meet this requirement, more rapid DNA analysis methods will be needed. Analytical methods based on single DNA molecules may replace the conventional DNA analysis procedure. This method also provides a more precise tool for studying interactions between DNA molecules and protein molecules. Single DNA can be prepared by the following operations: manipulation of genomic DNA, fixation in stretched form, mapping, cutting, recovery of the fragment, and PCR amplification. These operations can be conducted in a very small integrated analytical system made on a silicon substrate. The globular transformation can avoid breakdown of long genome DNA and permits manipulation of large DNA. Because the globular transition is reversible, the DNA can be pulled out sequentially from the globule of DNA and fixed on a substrate in an arbitrary pattern. This DNA molecule can be characterized by attaching a restriction enzyme or by probes. Eliminating the activity of the restriction enzyme by removing Mg ions, fiuorescence-labeled EcoRI is attached and an optical restriction map can then be made. Releasing Mg ions locally can then cut the DNA. The fragments can be recovered on a glass capillary using electrophoresis. The PCR method starting from an unknown single fragment of DNA molecule has been developed using a microreactor system based on a water-in-oil emulsion.Using this system, single DNA can be amplified. This system is suitable for manipulating and reacting single DNA molecules in an integrated analytical apparatus on a silicon substrate.KeywordsNeedle ElectrodeCoiled StateTrapping ForceLaser TrappingGlobular StateThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves." @default.
• W68477947 created "2016-06-24" @default.
• W68477947 creator A5056217295 @default.
• W68477947 date "2003-01-01" @default.
• W68477947 modified "2023-09-27" @default.
• W68477947 title "Manipulation of Single DNA Molecules" @default.
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• W68477947 doi "https://doi.org/10.1007/978-3-642-55503-9_3" @default.
• W68477947 hasPublicationYear "2003" @default.
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