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- W68484019 abstract "Several studies of molecular structure have successfully employed computer techniques to align images of single particles. Image processing software packages (e.g., P. R. Smith's MDPP system and J. Frank's SPIDER system) have also been developed to facilitate this work. Due to the low contrast and high beam damage rate involved in the use of unstained specimens, most single particle (i.e., non-crystalline) image alignment has been of negatively stained specimens. The scanning transmission electron microscope (STEM), which has a linear transfer function at high resolution rather than the oscillating one inherent to CEMs, operationally overcomes these limitations by providing low dose, high contrast images of unstained material with high collection efficiency." @default.
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- W68484019 date "1984-08-01" @default.
- W68484019 modified "2023-10-14" @default.
- W68484019 title "Alignment of low-dose STEM images of unstained macromolecules" @default.
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- W68484019 doi "https://doi.org/10.1017/s0424820100110957" @default.
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