Matches in SemOpenAlex for { <https://semopenalex.org/work/W68508775> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W68508775 endingPage "7" @default.
- W68508775 startingPage "353" @default.
- W68508775 abstract "DNA of Leptospira interrogans sv. lai strain 017 was labelled with digoxigenin or alpha 32P or biotin and used as probes to detect DNA of leptospires. Probes labelled with digoxigenin were able to detect 0.1-1 pg of homologous DNA and 10(2) of L. interrogans sv. lai strain 017. Probes alpha 32P and biotin detected 1 pg and 10 pg of homologous DNA and 10(3), 5 x 10(3) of L. interrogans sv. lai strain 017 respectively. These three probes couldn't detect L. biflexa sv. patoc strain Patoc I, L. illini strain 3055, Escherichia coli. Bacillus aerogenes capsulalus, Salmonella anatis, K-DNA of Leishmania, and of human WBC. Comparison of digoxigenin-, alpha 32P- and Biotin-labelled probes in the dot-blot hybridization assay on different serogroup sv. of leptospires revealed that digoxigenin-labelled probes were more sensitive than alpha 32P and biotin-labelled probes. The results indicate that digoxigenin-labelled probes DNA can be used for detection of leptospires in field and clinic. We also report procedures in situ hybridization with leptospira in tissues smear and plasma sediment of an experimentally infected guinea pig. It offers the advantage of recognizable of leptospiral morphology in combination with a hybridization signals. The results indicate that digoxigenin-labelled DNA probes of 017 strain might provide tool for routine diagnosis and classification in cases of leptospiral interrogans infection." @default.
- W68508775 created "2016-06-24" @default.
- W68508775 creator A5041264584 @default.
- W68508775 creator A5050021948 @default.
- W68508775 date "1992-09-01" @default.
- W68508775 modified "2023-09-23" @default.
- W68508775 title "[Marking and detection of DNA of leptospires in the dot-blot and situ hybridization with digoxigenin-labelled probes]." @default.
- W68508775 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1304535" @default.
- W68508775 hasPublicationYear "1992" @default.
- W68508775 type Work @default.
- W68508775 sameAs 68508775 @default.
- W68508775 citedByCount "0" @default.
- W68508775 crossrefType "journal-article" @default.
- W68508775 hasAuthorship W68508775A5041264584 @default.
- W68508775 hasAuthorship W68508775A5050021948 @default.
- W68508775 hasConcept C10389963 @default.
- W68508775 hasConcept C104317684 @default.
- W68508775 hasConcept C130197536 @default.
- W68508775 hasConcept C130840561 @default.
- W68508775 hasConcept C133307689 @default.
- W68508775 hasConcept C150194340 @default.
- W68508775 hasConcept C153911025 @default.
- W68508775 hasConcept C159047783 @default.
- W68508775 hasConcept C185592680 @default.
- W68508775 hasConcept C2776026663 @default.
- W68508775 hasConcept C2776552150 @default.
- W68508775 hasConcept C2778204606 @default.
- W68508775 hasConcept C2780542935 @default.
- W68508775 hasConcept C552990157 @default.
- W68508775 hasConcept C55493867 @default.
- W68508775 hasConcept C81439078 @default.
- W68508775 hasConcept C86803240 @default.
- W68508775 hasConceptScore W68508775C10389963 @default.
- W68508775 hasConceptScore W68508775C104317684 @default.
- W68508775 hasConceptScore W68508775C130197536 @default.
- W68508775 hasConceptScore W68508775C130840561 @default.
- W68508775 hasConceptScore W68508775C133307689 @default.
- W68508775 hasConceptScore W68508775C150194340 @default.
- W68508775 hasConceptScore W68508775C153911025 @default.
- W68508775 hasConceptScore W68508775C159047783 @default.
- W68508775 hasConceptScore W68508775C185592680 @default.
- W68508775 hasConceptScore W68508775C2776026663 @default.
- W68508775 hasConceptScore W68508775C2776552150 @default.
- W68508775 hasConceptScore W68508775C2778204606 @default.
- W68508775 hasConceptScore W68508775C2780542935 @default.
- W68508775 hasConceptScore W68508775C552990157 @default.
- W68508775 hasConceptScore W68508775C55493867 @default.
- W68508775 hasConceptScore W68508775C81439078 @default.
- W68508775 hasConceptScore W68508775C86803240 @default.
- W68508775 hasIssue "4" @default.
- W68508775 hasLocation W685087751 @default.
- W68508775 hasOpenAccess W68508775 @default.
- W68508775 hasPrimaryLocation W685087751 @default.
- W68508775 hasRelatedWork W2007315677 @default.
- W68508775 hasRelatedWork W2021712506 @default.
- W68508775 hasRelatedWork W2047528330 @default.
- W68508775 hasRelatedWork W2049407302 @default.
- W68508775 hasRelatedWork W2070275042 @default.
- W68508775 hasRelatedWork W2106876212 @default.
- W68508775 hasRelatedWork W2126347872 @default.
- W68508775 hasRelatedWork W2359925301 @default.
- W68508775 hasRelatedWork W68508775 @default.
- W68508775 hasRelatedWork W9748801 @default.
- W68508775 hasVolume "23" @default.
- W68508775 isParatext "false" @default.
- W68508775 isRetracted "false" @default.
- W68508775 magId "68508775" @default.
- W68508775 workType "article" @default.