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- W6852720 abstract "Upon detergent or hypo-osmotic lysis of CHO-cell postnuclear supernatants or isolated lysosomes at pH 4.8, the lysosomal enzymes beta-hexosaminidase, beta-galactosidase, alpha-fucosidase and cathepsin C were readily pelleted, whereas the exogenous marker, long-term-internalized horseradish peroxidase, was not. Salt or pH elevation greatly decreased lysosomal-enzyme pelletability. The results suggest that, under native conditions, lysosomal hydrolases may be aggregated. Aggregation could promote enzyme retention within the organelle." @default.
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- W6852720 date "1988-02-01" @default.
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- W6852720 title "Effects of pH, detergent and salt on aggregation of Chinese-hamster-ovary-cell lysosomal enzymes" @default.
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- W6852720 doi "https://doi.org/10.1042/bj2490921" @default.
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