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- W68761646 abstract "Despite their size, microbes manage to exert profound effects on the human macroworld. Traditionally, the field of microbiology has been focused at the population level, limited primarily by the resolution of its workhorse technologies – growth after inoculation into liquid or solid media. However, such “bulk-phase” or population-scale measurements are composed of data from thousands or millions of individual cellular inputs. Averaging the measured parameter across the population may “smooth over” and obscure information on discrete cellular phenomena that may otherwise provide key insights into how microbial cells function or interact with their environments. As alternatives to population-based methods, new tools are needed to visualize discrete microbial structures or phenomena that are inaccessible via traditional means. Classically defined, “imaging” refers chiefly to methods that use visible light to generate a likeness of an object. However, approaches using other regions of the electromagnetic spectrum or nonphotonic processes can also be used to generate graphic representations of microbial cells or provide quantitative measurements, often capturing details not available via other means. Examples include Raman microspectroscopy, microbeam analyses and atomic force microscopy. This chapter seeks to provide a cross-sectional sampling of the diversity of new technologies for imaging and analysis of individual microbial cells and how they have been applied to the problems of single-cell microbiology. Special attention is paid to commercially available technologies allowing dynamic observation of discrete phenomena occurring within individual living microbial cells." @default.
- W68761646 created "2016-06-24" @default.
- W68761646 creator A5038319927 @default.
- W68761646 date "2007-01-01" @default.
- W68761646 modified "2023-09-23" @default.
- W68761646 title "New Technologies for Imaging and Analysis of Individual Microbial Cells" @default.
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