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- W69160093 abstract "Structure of the microbody crystalloid in yeast Kloeckera sp. no. 2201 was studied by ultrathin frozen sectioning electron microscopy with optical diffraction and filtering methods. The crystalloid showed cubic lattice structure with a unit cell length of 22 nm and an angle of 90°. The lattice was composed of two types of protein units different in size. The larger unit with a diameter of 10 nm was in the tetragonal form, having apparent four-fold symmetry. The smaller unit, 7 nm in diameter, appeared to have two parallel short bars with two-fold symmetry. Knowing the molecular weight and the specific enzyme activity of the two proteins, they were identified as alcohol oxidase and catalase, respectively. This leads us to the conclusion that the microbody crystalloid is a composite crystal of these two enzymes, each being spaced alternately in a cubic lattice. KEYWORDS Microbody crystalloid; Kloeckera microbodies; utilization of methanol; composite crystal; ultrathin frozen sectioning" @default.
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- W69160093 date "1981-01-01" @default.
- W69160093 modified "2023-09-23" @default.
- W69160093 title "STRUCTURE OF THE MICROBODY CRYSTALLOID IN METHANOL GROWN YEASTS AS A COMPOSITE CRYSTAL OF CATALASE AND ALCOHOL OXIDASE" @default.
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- W69160093 doi "https://doi.org/10.1016/b978-0-08-025382-4.50016-2" @default.
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