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- W69270532 abstract "Summary - Embryogenic callus was induced with hypocotyls of Gossypium hirsutum L. cv Henan 79, Simian 2, Lumian 6 and Coker 312. Suspension cell cultures were established in four genotypes. In a cytological study, 6-month cell cultures of Coker 312 were analyzed and revealed to be variable in chromosome number and structure. The cell cultures showed a wide range of chromosome numbers, varying from diploid to hexaploid in euploid and from 16 to more than 78 in aneuploid lines. The normal tetraploid cells were found only at 21.01% frequency. Several mitotic abnormalities were found, such as multi-nuclei and micronucleus in interphase; chromosome rings and telophase bridges in the mitotic stage. Only 85.50% of the cells of the suspension cultures were found to be normal with a single interphase nucleus; 3.30% had two and 0.63% had four interphase nuclei. The variation in chromosomes tended to increase with the time in culture. The variability of chromosomes in cell cultures seems to be one of the main reasons for failure of embryo development." @default.
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- W69270532 date "1992-01-01" @default.
- W69270532 modified "2023-10-16" @default.
- W69270532 title "CHROMOSOME VARIATION IN CALLUS CULTURE OF GOSSYPIUM HIRSUTUM L." @default.
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- W69270532 doi "https://doi.org/10.1016/b978-0-12-044140-2.50017-x" @default.
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