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• W69571828 abstract "Research Article1 April 1994free access The HimA and HimD subunits of integration host factor can specifically bind to DNA as homodimers. L. Zulianello L. Zulianello Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands. Search for more papers by this author E. de la Gorgue de Rosny E. de la Gorgue de Rosny Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands. Search for more papers by this author P. van Ulsen P. van Ulsen Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands. Search for more papers by this author P. van de Putte P. van de Putte Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands. Search for more papers by this author N. Goosen N. Goosen Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands. Search for more papers by this author L. Zulianello L. Zulianello Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands. Search for more papers by this author E. de la Gorgue de Rosny E. de la Gorgue de Rosny Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands. Search for more papers by this author P. van Ulsen P. van Ulsen Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands. Search for more papers by this author P. van de Putte P. van de Putte Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands. Search for more papers by this author N. Goosen N. Goosen Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands. Search for more papers by this author Author Information L. Zulianello1, E. Gorgue de Rosny1, P. Ulsen1, P. Putte1 and N. Goosen1 1Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands. The EMBO Journal (1994)13:1534-1540https://doi.org/10.1002/j.1460-2075.1994.tb06415.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Integration host factor (IHF) is a heterodimeric protein from Escherichia coli which specifically binds to an asymmetric consensus sequence. We have isolated the individual subunits of IHF, HimA and HimD, and show that an active IHF protein can be reconstituted from these subunits. The HimA and HimD polypeptides alone are capable of specifically recognizing the same ihf sequence. The mobilities of the protein-DNA complexes in a gel-retardation assay suggest that the proteins bind as homodimers. The stability of the HimD-DNA complex is approximately 100-fold lower than that of the IHF-DNA complex. The HimA-DNA complex is even less stable and is only observed when a large excess of HimA is used. This instability is possibly due to the inability of HimA to form stable homodimers. By domain swapping between HimA and HimD, we have constructed an IHF fusion protein which has the putative DNA-binding domains of only HimA. This fusion protein forms stable dimers and makes specific protein-DNA complexes with a high efficiency. A comparable fusion protein with only the DNA-binding domains of HimD forms less stable complexes, suggesting that sequence-specific contacts between IHF and the ihf consensus are mainly provided by the HimA subunit. Previous ArticleNext Article Volume 13Issue 71 April 1994In this issue RelatedDetailsLoading ..." @default.
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• W69571828 title "The HimA and HimD subunits of integration host factor can specifically bind to DNA as homodimers." @default.
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• W69571828 doi "https://doi.org/10.1002/j.1460-2075.1994.tb06415.x" @default.
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