Matches in SemOpenAlex for { <https://semopenalex.org/work/W69738491> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W69738491 abstract "After bleaching of cultured cells labelled with a fluorescent dye with laser light, one observes fluorescence recovery due to lateral motion of dye molecules from adjacent unbleached domains (Fluorescence Recovery After Photobleaching, FRAP). In the usual FRAP systems, the laser beam is directed vertically onto the cells, hence one measures the average fluorescence kinetics of all membranes throughout the full depth of the cell. This experimental design was modified by an inclination of the laser beam at an oblique angle, which was selected in such a manner that total reflection of the laser light near the cell surface resulted (Total Internal Reflection Fluorescence Microscopy, TIRF). Despite of TIRF, the irradiated region is bleached because a small fraction of the beam energy is transferred into the cell, which exponentially decays to values near zero already within a penetration depth in the order of magnitude of 50-150 nm. This combination of FRAP and TIRF for the study of living cells is new and permits a largely selective measurement of the fluorescence kinetics in the outer cell membrane, due to the selective superficial bleaching process. As the theory of the classical design is not fully appropriate in the present context, a generalized theoretical model of FRAP was developed which considers angular-dependent effects. Experiments on lateral mobility of the low molecular weight lipid analogon Nile Red (9-Diethylamino-5H-benzo[a]phenoxazin-5-on) on confluent cultures of HeLa cells were performed at bleaching periods of 200–1000 msec with an Argon laser of wavelength 488 nm. From the resulting data, we could quantify the speed of diffusion in terms of time for half recovery of the fluorescence intensity, determine whether the investigated molecule was completely mobile or partially fixed to the membrane, and explore whether its lateral mobility is due to diffusion only or also to active transport mechanisms." @default.
- W69738491 created "2016-06-24" @default.
- W69738491 creator A5027353075 @default.
- W69738491 creator A5058923912 @default.
- W69738491 creator A5075662161 @default.
- W69738491 creator A5079889652 @default.
- W69738491 creator A5086468982 @default.
- W69738491 date "1994-01-01" @default.
- W69738491 modified "2023-09-23" @default.
- W69738491 title "Fluorescence Recovery after Photobleaching Studied by Total Internal Reflection Microscopy: An Experimental System for Studies on Living Cells in Culture" @default.
- W69738491 cites W1556700452 @default.
- W69738491 cites W1991655565 @default.
- W69738491 cites W1992722956 @default.
- W69738491 cites W2017934591 @default.
- W69738491 cites W2028808142 @default.
- W69738491 cites W2037661488 @default.
- W69738491 cites W2051509280 @default.
- W69738491 cites W2051542254 @default.
- W69738491 cites W2056847422 @default.
- W69738491 doi "https://doi.org/10.1007/978-3-0348-8501-0_32" @default.
- W69738491 hasPublicationYear "1994" @default.
- W69738491 type Work @default.
- W69738491 sameAs 69738491 @default.
- W69738491 citedByCount "1" @default.
- W69738491 crossrefType "book-chapter" @default.
- W69738491 hasAuthorship W69738491A5027353075 @default.
- W69738491 hasAuthorship W69738491A5058923912 @default.
- W69738491 hasAuthorship W69738491A5075662161 @default.
- W69738491 hasAuthorship W69738491A5079889652 @default.
- W69738491 hasAuthorship W69738491A5086468982 @default.
- W69738491 hasConcept C111337013 @default.
- W69738491 hasConcept C113196181 @default.
- W69738491 hasConcept C120665830 @default.
- W69738491 hasConcept C121332964 @default.
- W69738491 hasConcept C121381206 @default.
- W69738491 hasConcept C12554922 @default.
- W69738491 hasConcept C147080431 @default.
- W69738491 hasConcept C151730666 @default.
- W69738491 hasConcept C169274487 @default.
- W69738491 hasConcept C170395517 @default.
- W69738491 hasConcept C185544564 @default.
- W69738491 hasConcept C185592680 @default.
- W69738491 hasConcept C192562407 @default.
- W69738491 hasConcept C193493375 @default.
- W69738491 hasConcept C26659971 @default.
- W69738491 hasConcept C2779343474 @default.
- W69738491 hasConcept C31332276 @default.
- W69738491 hasConcept C43617362 @default.
- W69738491 hasConcept C520434653 @default.
- W69738491 hasConcept C86803240 @default.
- W69738491 hasConcept C91881484 @default.
- W69738491 hasConceptScore W69738491C111337013 @default.
- W69738491 hasConceptScore W69738491C113196181 @default.
- W69738491 hasConceptScore W69738491C120665830 @default.
- W69738491 hasConceptScore W69738491C121332964 @default.
- W69738491 hasConceptScore W69738491C121381206 @default.
- W69738491 hasConceptScore W69738491C12554922 @default.
- W69738491 hasConceptScore W69738491C147080431 @default.
- W69738491 hasConceptScore W69738491C151730666 @default.
- W69738491 hasConceptScore W69738491C169274487 @default.
- W69738491 hasConceptScore W69738491C170395517 @default.
- W69738491 hasConceptScore W69738491C185544564 @default.
- W69738491 hasConceptScore W69738491C185592680 @default.
- W69738491 hasConceptScore W69738491C192562407 @default.
- W69738491 hasConceptScore W69738491C193493375 @default.
- W69738491 hasConceptScore W69738491C26659971 @default.
- W69738491 hasConceptScore W69738491C2779343474 @default.
- W69738491 hasConceptScore W69738491C31332276 @default.
- W69738491 hasConceptScore W69738491C43617362 @default.
- W69738491 hasConceptScore W69738491C520434653 @default.
- W69738491 hasConceptScore W69738491C86803240 @default.
- W69738491 hasConceptScore W69738491C91881484 @default.
- W69738491 hasLocation W697384911 @default.
- W69738491 hasOpenAccess W69738491 @default.
- W69738491 hasPrimaryLocation W697384911 @default.
- W69738491 hasRelatedWork W146707336 @default.
- W69738491 hasRelatedWork W1970949493 @default.
- W69738491 hasRelatedWork W1972346753 @default.
- W69738491 hasRelatedWork W1985928912 @default.
- W69738491 hasRelatedWork W1988401928 @default.
- W69738491 hasRelatedWork W2015147882 @default.
- W69738491 hasRelatedWork W2017934591 @default.
- W69738491 hasRelatedWork W2039276239 @default.
- W69738491 hasRelatedWork W2052697551 @default.
- W69738491 hasRelatedWork W2056847422 @default.
- W69738491 hasRelatedWork W2057994985 @default.
- W69738491 hasRelatedWork W2061900255 @default.
- W69738491 hasRelatedWork W2070649499 @default.
- W69738491 hasRelatedWork W2077529654 @default.
- W69738491 hasRelatedWork W2078800834 @default.
- W69738491 hasRelatedWork W2080661489 @default.
- W69738491 hasRelatedWork W2094720589 @default.
- W69738491 hasRelatedWork W23845359 @default.
- W69738491 hasRelatedWork W2789311093 @default.
- W69738491 hasRelatedWork W2902959876 @default.
- W69738491 isParatext "false" @default.
- W69738491 isRetracted "false" @default.
- W69738491 magId "69738491" @default.
- W69738491 workType "book-chapter" @default.