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- W69830667 abstract "Publisher Summary For generations rinderpest was diagnosed purely by observation of clinical signs. This chapter provides an overview of the current diagnostic methods used to detect and differentiate between rinderpest and PPR infections in ruminant species. Both antibody and antigen detection methods are described, the former used extensively for evaluating the success of vaccination campaigns and serosurveillance, and the latter for the detection of virus in clinical samples. The polymerase chain reaction and nucleotide sequencing allows a rapid diagnostic result, which can identify the lineage of the virus concerned and give a clear picture of the epidemiological situation. One of the earliest diagnostic techniques for the detection of rinderpest virus antigens was the agar gel immunodiffusion test. The technique was greatly improved when high titre hyperimmune rabbit serum was introduced. This was produced using a protocol, which did not induce antibodies to cellular proteins thus reducing non-specific reactions. The advent of monoclonal antibodies made possible accurate differential diagnosis of many virus diseases. Monoclonal antibody profiling can be used to differentiate between RPV and PPRV, however, enzyme-linked immunosorbent assay technology offered the opportunity to develop simple, rapid, and highly specific diagnostic assays to identify RPV and PPRV. These tests are carried out in 96-well plates and are suitable for large-scale sample analyses." @default.
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- W69830667 date "2006-01-01" @default.
- W69830667 modified "2023-09-26" @default.
- W69830667 title "Diagnosis of rinderpest virus and peste des petits ruminants virus" @default.
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- W69830667 doi "https://doi.org/10.1016/b978-012088385-1/50038-1" @default.
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