Matches in SemOpenAlex for { <https://semopenalex.org/work/W69884084> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W69884084 abstract "The establishment of apico-basal polarity and lumen formation are fundamental stepsduring vertebrate neural tube development. In zebrafish embryos, the lumen is generatedde novo from a solid rod structure. Prior to this, the neural cells acquire apico-basalpolarity when they undergo mirror-symmetric division and one daughter cell crosses themidline (C‐division). This specialised division must be tightly regulated in time and spacefor correct neural tube morphogenesis. I have shown that delaying convergence in wildtypetissue results in C‐divisions occurring at the right time but in the wrong place and thegeneration of neural tube duplications. This uncoupling of individual cell behaviours fromtissue morphogenesis suggests that an intrinsic timer controls cell polarisation and Cdivisionduring neurulation.I have tested this hypothesis by heterochronic cell transplantation. I have found that bothyounger and older transplanted cells polarise according to their age, show a decreasedability to cross the midline during neurulation, and that older cells can generate ectopiclumens within the host neuroepithelium. These results all support the idea that anintrinsic timer controls cell polarisation and lumen formation. They suggest that the timerbegins even before cells are specified as neural, and can resist many environmental signalsduring gastrulation and neurulation that could reset it.I have also investigated the role of the environment in neural cell polarisation byexamining the establishment of polarity of cells in embryos with abnormal neural tubemorphogenesis, and in extreme ectopic locations both in the embryo and in 3D culture.Cells can polarise in all these environments, although polarisation can be delayed by 1hourby severe morphogenetic defects. Lastly I have found that cells do not monitor time bycounting the number of cell cycles and that cell polarisation does not even require mirror-symmetriccell division. Overall my results highlight the importance of mechanisms thatcoordinate cell intrinsic programmes with morphogenetic movements and environmentalsignals, both in time and space, for correct embryogenesis." @default.
- W69884084 created "2016-06-24" @default.
- W69884084 creator A5032022564 @default.
- W69884084 date "2010-09-01" @default.
- W69884084 modified "2023-09-27" @default.
- W69884084 title "Investigating the mechanisms that regulate the establishment of polarity during zebrafish neural tube formation" @default.
- W69884084 hasPublicationYear "2010" @default.
- W69884084 type Work @default.
- W69884084 sameAs 69884084 @default.
- W69884084 citedByCount "0" @default.
- W69884084 crossrefType "dissertation" @default.
- W69884084 hasAuthorship W69884084A5032022564 @default.
- W69884084 hasConcept C102225024 @default.
- W69884084 hasConcept C103607123 @default.
- W69884084 hasConcept C104317684 @default.
- W69884084 hasConcept C105702510 @default.
- W69884084 hasConcept C129536746 @default.
- W69884084 hasConcept C136834591 @default.
- W69884084 hasConcept C1491633281 @default.
- W69884084 hasConcept C196843134 @default.
- W69884084 hasConcept C197849852 @default.
- W69884084 hasConcept C200707615 @default.
- W69884084 hasConcept C2776878037 @default.
- W69884084 hasConcept C2777361361 @default.
- W69884084 hasConcept C2780594696 @default.
- W69884084 hasConcept C28328180 @default.
- W69884084 hasConcept C40692019 @default.
- W69884084 hasConcept C49353375 @default.
- W69884084 hasConcept C54355233 @default.
- W69884084 hasConcept C85813293 @default.
- W69884084 hasConcept C86803240 @default.
- W69884084 hasConcept C87073359 @default.
- W69884084 hasConcept C95444343 @default.
- W69884084 hasConceptScore W69884084C102225024 @default.
- W69884084 hasConceptScore W69884084C103607123 @default.
- W69884084 hasConceptScore W69884084C104317684 @default.
- W69884084 hasConceptScore W69884084C105702510 @default.
- W69884084 hasConceptScore W69884084C129536746 @default.
- W69884084 hasConceptScore W69884084C136834591 @default.
- W69884084 hasConceptScore W69884084C1491633281 @default.
- W69884084 hasConceptScore W69884084C196843134 @default.
- W69884084 hasConceptScore W69884084C197849852 @default.
- W69884084 hasConceptScore W69884084C200707615 @default.
- W69884084 hasConceptScore W69884084C2776878037 @default.
- W69884084 hasConceptScore W69884084C2777361361 @default.
- W69884084 hasConceptScore W69884084C2780594696 @default.
- W69884084 hasConceptScore W69884084C28328180 @default.
- W69884084 hasConceptScore W69884084C40692019 @default.
- W69884084 hasConceptScore W69884084C49353375 @default.
- W69884084 hasConceptScore W69884084C54355233 @default.
- W69884084 hasConceptScore W69884084C85813293 @default.
- W69884084 hasConceptScore W69884084C86803240 @default.
- W69884084 hasConceptScore W69884084C87073359 @default.
- W69884084 hasConceptScore W69884084C95444343 @default.
- W69884084 hasLocation W698840841 @default.
- W69884084 hasOpenAccess W69884084 @default.
- W69884084 hasPrimaryLocation W698840841 @default.
- W69884084 hasRelatedWork W1566052773 @default.
- W69884084 hasRelatedWork W16679824 @default.
- W69884084 hasRelatedWork W172598045 @default.
- W69884084 hasRelatedWork W1985661051 @default.
- W69884084 hasRelatedWork W2022377852 @default.
- W69884084 hasRelatedWork W2072953590 @default.
- W69884084 hasRelatedWork W2076047783 @default.
- W69884084 hasRelatedWork W2085062185 @default.
- W69884084 hasRelatedWork W2104324482 @default.
- W69884084 hasRelatedWork W2108239878 @default.
- W69884084 hasRelatedWork W2146120609 @default.
- W69884084 hasRelatedWork W2152829771 @default.
- W69884084 hasRelatedWork W2412277739 @default.
- W69884084 hasRelatedWork W2501070998 @default.
- W69884084 hasRelatedWork W2557119088 @default.
- W69884084 hasRelatedWork W2564002198 @default.
- W69884084 hasRelatedWork W2791712385 @default.
- W69884084 hasRelatedWork W3111631623 @default.
- W69884084 hasRelatedWork W358499892 @default.
- W69884084 hasRelatedWork W3092678834 @default.
- W69884084 isParatext "false" @default.
- W69884084 isRetracted "false" @default.
- W69884084 magId "69884084" @default.
- W69884084 workType "dissertation" @default.