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- W69895723 abstract "Publisher Summary This chapter discusses the in situ hybridization for neurofilament and GAP-43 mRNA in primary sensory neurons in conjunction with nerve growth factor (NGF) receptor radioautography. The methods described in this chapter were developed to compare the responses to injury and NGF infusion of neurons with and without the ability to respond to NGF. Labeling of DRG neurons was quantified in radioautographs prepared after incubation of tissue sections with 40 pM 125 I-labeled NGF or in situ hybridization with a cDNA probe for neurofilament medium subunit (NFM) or GAP-43 (growth-associated protein). Because of different requirements for fixation, receptor radioautography and in situ hybridization cannot be performed on the same section. Therefore, each of two adjacent sections was processed by one or the other technique and individual neurons were identified and quantified in such pairs of sections." @default.
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- W69895723 date "1992-01-01" @default.
- W69895723 modified "2023-09-24" @default.
- W69895723 title "In Situ Hybridization for Neurofilament and GAP-43 mRNA in Primary Sensory Neurons in Conjunction with Nerve Growth Factor Receptor Radioautography" @default.
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- W69895723 doi "https://doi.org/10.1016/b978-0-12-185267-2.50023-4" @default.
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