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- W70937614 abstract "Detection of donor-specific anti-HLA antibodies (DSA) by solid phase assay (SpA) helps donor selection by predicting the actual crossmatch (xM), a process referred as a virtual crossmatch (VxM). At our center we find good correlation (R2 = 0.85) between flow xM (FxM) and VxM using single antigen bead (SAB) assay to identify DSA. The SAB assay is considered a sensitive SpA; however, a concern is that clinically insignificant anti-HLA antibodies (HLA-Ab) may be detected and preclude the recipient from receiving a transplant. The complement fixing (C1q) SAB assay has been proposed as a way to minimize the detection of clinically insignificant HLA-Ab. We present a summary of our evaluation of this assay. 20 positive sera, previously used for serological typing, were tested with SAB, at 1:8 dilution with SAB and by C1q SAB method as per the manufacturer’s protocol. Serological HLA-Ab specificities were compared with those that were obtained by SAB and C1q SAB. For the SpA, specificities >5000 MFI were considered as positive. Specificities were grouped into two categories: 5000–10,000 MFI and >10,000 MFI as in our center >5000 MFI correlates with a positive FxM and >10,000 MFI correlates with cytotoxicity xM. In total there were 53 HLA-Ab specificities as defined by serology, 328 as defined by SAB, and 123 as defined by C1q. SAB identified all 53 specificities defined by serology; 49 with MFI >10,000. Of the 4 with MFI 10,000 on 1:8 dilution and other 2 were not detected by C1q assay. In addition, C1q assay did not detect 3 specificities identified by serology (all with MFI >10,000 by SAB). C1q assay identified 123/179 (69%) of the specificities with MFI >10,000 and 0/151 specificities with MFI: 5000–10,000 as detected by SAB (table). For Class I HLA-Ab, C1q assay only detects high titer/avidity antibodies as defined by SAB. As DSA with SAB MFI: 5000–10,000 can result in a positive FxM, additional investigation is warranted to determine the clinical significance of these HLA-Ab. Serology positiveN%SABTotal positive53100MFI: >10,0004992MFI: 5000–10,00048Total negative00C1q SABTotal positive4789MFI: >10,0004789MFI: 5000–10,00000Total negative511 M.J. Gandhi: Consultant; Company/Organization; Thermo Fisher-One Lambda." @default.
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- W70937614 date "2014-10-01" @default.
- W70937614 modified "2023-10-18" @default.
- W70937614 title "P036" @default.
- W70937614 doi "https://doi.org/10.1016/j.humimm.2014.08.098" @default.
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