FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W71028032> ?p ?o ?g. }

• W71028032 abstract "Using human DAG lipase α and β specific RT-PCR, we identified two alternatively spliced variants of human DAG lipase mRNAs, DAG lipase α–1 and β-1, in human multiple tissues. The alternative splicing of DAG lipase α-1 occurred at +206 (Gene bank: NM_006133), spliced out 212 bp (+206 to +418 bp) of the coding region, and encoded a 956 amino acid DAG lipase α-1 protein. The N-terminus of DAG lipase α–1 differed from that of DAG lipase α. The alternative splicing of DAG lipase β-1 occurred at 360 (Gene bank: NM_139179), spliced out 552 bp (+360 to +912 bp) of the coding region, and encoded a 391 amino acid DAG lipase β-1 protein with a 281 amino acid N-terminal deletion. This study is the first to document alternative splicing of DAG lipase genes. We therefore studied DAG lipase α-1 and DAG lipase β-1 mRNA distribution in human tissues (heart, kidney, brain, lung, liver, spleen, and small intestine). DAG lipase α-1 mRNA was highly expressed in heart, kidney, brain, liver, spleen, small intestine and smooth muscle, but not lung. DAG lipase β-1 mRNA was highly expressed in kidney, brain, spleen, small intestine and smooth muscle, but not heart, liver, and lung. DAG lipase α-1 and DAG lipase β-1 were not found in rat and mouse tissues, indicating species specificity of the alternative splicing variants of DAG lipase mRNA expression. When recombinant DAG lipase α-1 and DAG lipase β-1 were expressed in HEK293/EBNA cells, DAG lipase α-1 was present almost exclusively in membrane, whereas DAG lipase β-1 was present in cytosolic fractions. The data suggest that the N-terminus of DAG lipases may be important for this protein translocating to the cell membrane, and alternative splicing variants of DAG lipase α or β may play an unique role in the regulation of “on-demand” 2-AG production in human tissues." @default.
• W71028032 created "2016-06-24" @default.
• W71028032 creator A5032447166 @default.
• W71028032 creator A5039431549 @default.
• W71028032 creator A5063264264 @default.
• W71028032 creator A5064256590 @default.
• W71028032 date "2008-03-01" @default.
• W71028032 modified "2023-10-16" @default.
• W71028032 title "Identification of the DAG Lipase alpha‐1 and beta‐1 mRNA transcripts in Human Tissues." @default.
• W71028032 doi "https://doi.org/10.1096/fasebj.22.1_supplement.791.4" @default.
• W71028032 hasPublicationYear "2008" @default.
• W71028032 type Work @default.
• W71028032 sameAs 71028032 @default.
• W71028032 citedByCount "0" @default.
• W71028032 crossrefType "journal-article" @default.
• W71028032 hasAuthorship W71028032A5032447166 @default.
• W71028032 hasAuthorship W71028032A5039431549 @default.
• W71028032 hasAuthorship W71028032A5063264264 @default.
• W71028032 hasAuthorship W71028032A5064256590 @default.
• W71028032 hasConcept C104317684 @default.
• W71028032 hasConcept C105580179 @default.
• W71028032 hasConcept C153911025 @default.
• W71028032 hasConcept C181199279 @default.
• W71028032 hasConcept C194583182 @default.
• W71028032 hasConcept C195794163 @default.
• W71028032 hasConcept C2779697368 @default.
• W71028032 hasConcept C2780902120 @default.
• W71028032 hasConcept C36020004 @default.
• W71028032 hasConcept C55493867 @default.
• W71028032 hasConcept C86803240 @default.
• W71028032 hasConcept C94879076 @default.
• W71028032 hasConceptScore W71028032C104317684 @default.
• W71028032 hasConceptScore W71028032C105580179 @default.
• W71028032 hasConceptScore W71028032C153911025 @default.
• W71028032 hasConceptScore W71028032C181199279 @default.
• W71028032 hasConceptScore W71028032C194583182 @default.
• W71028032 hasConceptScore W71028032C195794163 @default.
• W71028032 hasConceptScore W71028032C2779697368 @default.
• W71028032 hasConceptScore W71028032C2780902120 @default.
• W71028032 hasConceptScore W71028032C36020004 @default.
• W71028032 hasConceptScore W71028032C55493867 @default.
• W71028032 hasConceptScore W71028032C86803240 @default.
• W71028032 hasConceptScore W71028032C94879076 @default.
• W71028032 hasIssue "S1" @default.
• W71028032 hasLocation W710280321 @default.
• W71028032 hasOpenAccess W71028032 @default.
• W71028032 hasPrimaryLocation W710280321 @default.
• W71028032 hasRelatedWork W1552200847 @default.
• W71028032 hasRelatedWork W1994047703 @default.
• W71028032 hasRelatedWork W1996595158 @default.
• W71028032 hasRelatedWork W2009810944 @default.
• W71028032 hasRelatedWork W2042929604 @default.
• W71028032 hasRelatedWork W2061776195 @default.
• W71028032 hasRelatedWork W2064910354 @default.
• W71028032 hasRelatedWork W2086163952 @default.
• W71028032 hasRelatedWork W2109583711 @default.
• W71028032 hasRelatedWork W2113355817 @default.
• W71028032 hasVolume "22" @default.
• W71028032 isParatext "false" @default.
• W71028032 isRetracted "false" @default.
• W71028032 magId "71028032" @default.
• W71028032 workType "article" @default.