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• W71150101 abstract "There is great interest in both the medical and scientific communities in submicron cell-derived particles termed microparticles or microvesicles. A great difficulty in this field, however, has been the optimization and standardization of techniques to measure these small particles. Although competing techniques have been developed, flow cytometry remains the dominant approach. However, the hurdle in analysis has always been the ability to accurately measure the size characteristics of small particles. Instrumentation was designed around whole blood analysis and, therefore, cellular measurements above 3um. Particles detected below the 3um “threshold” were considered to be debris. Due to advances in microscopy and the ability to identify the existence of less than 1um cellular particles, flow cytometry instrumentation has been developed to have the ability to identify populations from 200nm to 1um. In conjunction with advancements in hardware and instrument specific protocols, a specific range of controls have become available commercially for instrument optimization and standardization. These microbeads serve as a reference point for experimental replication and standardization, and by using beads of differing sizes and fluorescent intensities, one can optimize the flow cytometer for cellular analysis.In flow cytometry, standardization is essential for achieving consistent results and generating comparable data. The program designed should be comprehensive, encompassing reagents, protocols, instrument configuration, and, for qualitative analyses, fluorescence intensity units. In addition, the general principles and techniques should be applicable to similar instrumentation. Therefore, we propose a methodology for flow cytometric set-up, quality control (QC), and standardization using Dragon Beads (Bangs Laboratories, Inc.) at the following sizes for later cell tracking: 191nm, 520nm, 780nm, and 1.01um. By using beads of differing sizes and fluorescent intensities, one can optimize the flow cytometer for cellular analysis. Therefore, all voltages, gains, and threshold settings were optimized for Dragon Green Beads, Microparticles, and Platelets." @default.
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• W71150101 date "2012-01-01" @default.
• W71150101 modified "2023-09-27" @default.
• W71150101 title "Standardization of Flow Cytometry Instrumentation for the Analysis of Microparticles" @default.
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