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- W7133242 abstract "This chapter discusses whether some zymogen granules are capable of storing only one type of protein or whether certain types of proteins and enzymes are stored preferably. The chapter presents a study in which female albino rats were starved for 24 hours and bled by cutting the femoral artery. The parotid glands were removed and 2.5 g of minced parotid tissue was homogenized in a 22.5 ml cold homogenizing solution. Upward elution was carried out at room temperature. The flow rate was 1.3 ml/ min, and the fraction volume was kept at 9.1 ml. The reliability and simplicity of gel filtration have made it one of the most useful separation techniques in biochemistry. This technique is also suitable for isolation of biological membranes, receptors, and viruses. However, this method is unsuitable for the direct separation of granule populations." @default.
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- W7133242 date "1981-01-01" @default.
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- W7133242 title "PREPARATION OF ZYMOGEN GRANULES WITH GEL FILTRATION: DISTRIBUTION OF AMYLASE AND CARBONIC ANHYDRASE ACTIVITY FOLLOWING “LINEAR-DISCRETE” DENSITY GRADIENT CENTRIFUGATION" @default.
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- W7133242 doi "https://doi.org/10.1016/b978-0-08-027349-5.50029-x" @default.
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