Matches in SemOpenAlex for { <https://semopenalex.org/work/W7138350> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W7138350 abstract "Troponin C is a regulatory protein of the myofilament which binds to calcium to trigger the process of contraction. This protein exists in two isoforms, skeletal and cardiac, which are spatially and temporally regulated.Work in this project builds the primary stage of a long-term project, for using the gene transfer method to overexpress the skeletal isoform of Troponin C in cardiomyocytes. The long-term aim is to achieve complete or partial substitution of the native cardiac isoform and study the effects on contractile force produced, in normal and ischemic cardiomyocytes, both in vitro and in vivo.This project has involved designing, constructing and analyzing expression of adenoviral gene transfer vectors overexpressing the sTnC isoform. Several adenoviral vectors were generated with the wild type sTnC gene under the control of muscle-specific promoters. To facilitate analysis of protein expression and its subcellular localization, the sTnC protein was tagged with epitope tags and adenovirus generated, with this gene under the control of constitutive (CMV) and cardiac-specific (HCA) promoters. Epitope-tagged adenoviruses were expressed in vitro using mouse fibroblast (NIH3T3) cells and analyzed by western blot analysis, showing successful constitutive expression. Recombinant adenoviruses containing epitope-tagged-sTnC under the control of the human cardiac actin promoter showed cardiac-specific expression in cultured cardiomyocytes, in situ, using immunocytochemistry. The constitutively-expressing sTnC adenoviral vector showed successful expression in cardiomyocytes in culture, using northern blot analysis.A range of adenoviral vectors have been successfully generated, and constitutive and tissue-specific expression has been established for some of these vectors. Successes attained in this project have established the initial requirements to achieve the long-term goal to alter calcium sensitivity of myofilaments, by overexpression of sTnC isoform in cardiomyocytes, both in vitro and in vivo." @default.
- W7138350 created "2016-06-24" @default.
- W7138350 creator A5021103904 @default.
- W7138350 date "1998-01-01" @default.
- W7138350 modified "2023-09-26" @default.
- W7138350 title "Construction of recombinant adenoviruses encoding skeletal troponin C protein and expression analyses in transduced cardiac myocytes" @default.
- W7138350 hasPublicationYear "1998" @default.
- W7138350 type Work @default.
- W7138350 sameAs 7138350 @default.
- W7138350 citedByCount "1" @default.
- W7138350 crossrefType "dissertation" @default.
- W7138350 hasAuthorship W7138350A5021103904 @default.
- W7138350 hasConcept C104317684 @default.
- W7138350 hasConcept C150194340 @default.
- W7138350 hasConcept C153911025 @default.
- W7138350 hasConcept C159654299 @default.
- W7138350 hasConcept C195616568 @default.
- W7138350 hasConcept C2776415932 @default.
- W7138350 hasConcept C32470452 @default.
- W7138350 hasConcept C40767141 @default.
- W7138350 hasConcept C53345823 @default.
- W7138350 hasConcept C54355233 @default.
- W7138350 hasConcept C55493867 @default.
- W7138350 hasConcept C86803240 @default.
- W7138350 hasConcept C95444343 @default.
- W7138350 hasConceptScore W7138350C104317684 @default.
- W7138350 hasConceptScore W7138350C150194340 @default.
- W7138350 hasConceptScore W7138350C153911025 @default.
- W7138350 hasConceptScore W7138350C159654299 @default.
- W7138350 hasConceptScore W7138350C195616568 @default.
- W7138350 hasConceptScore W7138350C2776415932 @default.
- W7138350 hasConceptScore W7138350C32470452 @default.
- W7138350 hasConceptScore W7138350C40767141 @default.
- W7138350 hasConceptScore W7138350C53345823 @default.
- W7138350 hasConceptScore W7138350C54355233 @default.
- W7138350 hasConceptScore W7138350C55493867 @default.
- W7138350 hasConceptScore W7138350C86803240 @default.
- W7138350 hasConceptScore W7138350C95444343 @default.
- W7138350 hasLocation W71383501 @default.
- W7138350 hasOpenAccess W7138350 @default.
- W7138350 hasPrimaryLocation W71383501 @default.
- W7138350 hasRelatedWork W1507058068 @default.
- W7138350 hasRelatedWork W1557719847 @default.
- W7138350 hasRelatedWork W1775605207 @default.
- W7138350 hasRelatedWork W1974331564 @default.
- W7138350 hasRelatedWork W1975017803 @default.
- W7138350 hasRelatedWork W1985161348 @default.
- W7138350 hasRelatedWork W2015457919 @default.
- W7138350 hasRelatedWork W2030326532 @default.
- W7138350 hasRelatedWork W2034857585 @default.
- W7138350 hasRelatedWork W2052567042 @default.
- W7138350 hasRelatedWork W2052702817 @default.
- W7138350 hasRelatedWork W2054219704 @default.
- W7138350 hasRelatedWork W2085902727 @default.
- W7138350 hasRelatedWork W2090379218 @default.
- W7138350 hasRelatedWork W2139091946 @default.
- W7138350 hasRelatedWork W2169253011 @default.
- W7138350 hasRelatedWork W2356768272 @default.
- W7138350 hasRelatedWork W2410076027 @default.
- W7138350 hasRelatedWork W2474725460 @default.
- W7138350 hasRelatedWork W2973117475 @default.
- W7138350 isParatext "false" @default.
- W7138350 isRetracted "false" @default.
- W7138350 magId "7138350" @default.
- W7138350 workType "dissertation" @default.