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- W71680908 abstract "ChIP-Seq library preparation can be a challenging procedure to automate because of its low ChIP DNA input. This Application Note describes the successful automation of Illumina ChIP-Seq library preparation on the Eppendorf epMotion 5075 TMX, using the KAPA High-Throughput Library Preparation Kit from Kapa Biosystems. Size-selected libraries were prepared from as little as 1 ng of fragmented ChIP DNA. To obtain the recommended 100 ng of library material for sequencing on the Illumina Genome Analyzer IIx , only 18 amplification cycles were needed for 1 ng of input DNA, or 9 cycles when starting library construction with 10 ng of ChIP DNA." @default.
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- W71680908 date "2013-08-01" @default.
- W71680908 modified "2023-09-26" @default.
- W71680908 title "Automation of ChIP-Seq Library Preparation for Next Generation Sequencing on the epMotion® 5075 TMX" @default.
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- W71680908 doi "https://doi.org/10.2144/000114067" @default.
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