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- W71838544 abstract "Two different DNA methylase fractions were prepared from E.coli K-12 which methylate calf thymus DNA to a ratio of 5.0:1 and 0.4:1 of 6MAP/5MC, respectively. Using these fractions, the synthetic complementary deoxyhexanucleotides d (pC-A-T-C-A-T) 5′→3′ d (G-T-A-G-T-Ap) 3 ′ →5′ were investigated for the enzymatic methylation of adenine and cytosine in the duplex structure. The results show an interaction of the deoxyoligonucleotides in the double-stranded form with the donor of the methyl groups S-adenosyl-L-methionine (SAM) in the presence of the enzyme. Neither a methyl transfer to one of the bases nor free SAM could be detected. Thus we suggest the formation of a complex consisting of the deoxyoligonucleotides, SAM and enzyme which does not lead to methylation. At the polymer level DNA methylase activity was inhibited by homologous DNA which does not accept methyl groups. This can also be explained by a complex formation without succeeding methylation." @default.
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- W71838544 date "1980-01-01" @default.
- W71838544 modified "2023-09-25" @default.
- W71838544 title "ACTION OF DNA METHYLASE FROM ESCHERICHIA COLI K-12 ON THE COMPLEMENTARY DEOXYHEXANUCLEOTIDES d(pC-A-T-C-AT) AND d(G-T-A-G-T-Ap)" @default.
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- W71838544 doi "https://doi.org/10.1016/b978-0-08-025494-4.50024-3" @default.
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