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- W722593 abstract "Objective To prepare Sudan I-HS conjugates with the carrier protein for developing a new immune assay for Sudan I. Methods Sudan I was actived by succinic anhydride and converted into Sudan I hemisuccinate (Sudan I-HS) as the hapten, then Sudan I-HS was linked to carrier protein bovine serum albumin (BSA) as immunogen, and ovalbumin (OVA) as coating antigen all by the active ester method. Results The results of IR and HPLC-MS indicated that the hapten was synthesized successfully. The conjugation ratios of Sudan I-HS-BSA and Sudan I-HS-OVA confirmed by ultraviolet spectrum were 11 : 1 and 5 : 1, respectively. Conclusion Antigens Sudan I-HS-BSA and Sudan I-HS-OVA were prepared successfully." @default.
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- W722593 date "2008-05-01" @default.
- W722593 modified "2023-09-24" @default.
- W722593 title "Synthesis of Sudan I artificial antigen" @default.
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