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- W72332316 abstract "The solute carrier family SLC34 includes the sodium-phosphate cotransporter (NaPi) type IIa, b, c isomers in rodents and humans. The objective of this study was to identify the NaPi-II isomers in porcine viscera and to quantify the NaPi-II isomer mRNA in the gut of the weaned Yorkshire pig. We determined a 2.5-kb full cDNA sequence of NaPi-IIa, a 499-bp partial cDNA sequence of NaPi-IIb and a 268-bp partial cDNA sequence of NaPiIIc, which showed 89, 81 and 85% homology with the human isomer sequences, respectively. Tissue distribution analyses showed that NaPi-IIa mRNA was expressed only in kidney, NaPi-IIb mRNA only in lung and NaPi-IIc mRNA in intestine, kidney, lung, liver and heart. The NaPi-IIc mRNA level in the gut was quantified with real time RT-PCR (SmartCycler using the SYBR GreenI detection kit), showing that NaPi-IIc mRNA was low in the duodenum, highly abundant in the proximal jejunum and was present in a decreasing gradient (P<0.05) in the distal jejunum, ileum, cecum and colon. Pigs have a different expression pattern for the NaPi-II isomers in the viscera with Pi transported across the gut apical membrane by NaPi-IIc compared with rodents and humans." @default.
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- W72332316 date "2006-03-01" @default.
- W72332316 modified "2023-10-16" @default.
- W72332316 title "Visceral distribution of the type II sodium‐dependent phosphate cotransporter (NaPi‐II) isomer mRNA and the expression of NaPi‐IIc mRNA along the intestinal longitudinal axis in the post‐weaned pig" @default.
- W72332316 doi "https://doi.org/10.1096/fasebj.20.5.a1064-c" @default.
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