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- W72443592 abstract "Article1 August 1982 Origin of replication in chloroplast DNA of Euglena gracilis located close to the region of variable size Barbara Koller Barbara Koller European Molecular Biology Laboratory, Postfach 10.2209, D-6900 Heidelberg, FRG Search for more papers by this author Hajo Delius Hajo Delius European Molecular Biology Laboratory, Postfach 10.2209, D-6900 Heidelberg, FRG To whom reprint requests should be sent. Search for more papers by this author Barbara Koller Barbara Koller European Molecular Biology Laboratory, Postfach 10.2209, D-6900 Heidelberg, FRG Search for more papers by this author Hajo Delius Hajo Delius European Molecular Biology Laboratory, Postfach 10.2209, D-6900 Heidelberg, FRG To whom reprint requests should be sent. Search for more papers by this author Author Information Barbara Koller1 and Hajo Delius1 1European Molecular Biology Laboratory, Postfach 10.2209, D-6900 Heidelberg, FRG The EMBO Journal (1982)1:995-998https://doi.org/10.1002/j.1460-2075.1982.tb01283.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Chloroplast DNA (cpDNA), containing ˜10% replicative molecules, was isolated 2 h after onset of the dark period from cultures of Euglena gracilis strain Z. The DNA was digested with the restriction enzymes PvuII, SalI, BamHI, or EcoRI. Fragments that contained intact replicative loops were measured to determine the position of replicated sequences in relation to the restriction enzyme sites. It was found that replication starts at a unique position near one of the palindromic sequences I2 (Koller and Delius, 1982a) which is located upstream (with respect to the direction of rRNA transcription) of the AT-rich region of variable size (Jenni et al., 1981; Schlunegger et al., in preparation). In the majority of cases DNA synthesis proceeds unidirectionally away from this region for ˜5000 nucleotides before it starts in the other direction (in the same sense as the rRNA transcription) through the Z-region and the second palindromic sequence. Previous ArticleNext Article Volume 1Issue 81 August 1982In this issue RelatedDetailsLoading ..." @default.
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- W72443592 title "Origin of replication in chloroplast DNA of <i>Euglena gracilis</i> located close to the region of variable size" @default.
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- W72443592 doi "https://doi.org/10.1002/j.1460-2075.1982.tb01283.x" @default.
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