Matches in SemOpenAlex for { <https://semopenalex.org/work/W72497253> ?p ?o ?g. }
- W72497253 abstract "The expression of phospholipase C-β1 (PLC-β1) and cyclin D3 is highly induced during skeletalmyoblast differentiation. We have previously shown that PLC-β1 activates cyclin D3 promoter duringthe differentiation of myoblasts to myotubes, indicating that PLC-β1 is a crucial regulator of mousecyclin D3 gene. Here we report that PLC-β1 catalytic activity plays a role in the increase of cyclin D3levels and in the induction of differentiation of C2C12 skeletal muscle cells. PLC-β1 mutationalanalysis revealed the importance of His331 and His378 for the catalytic activity. We show that followinginsulin administration, cyclin D3 mRNA levels are lower in cells overexpressing the PLC-β1catalytically inactive form, as compared to wild type cells. We describe a novel signaling pathwayelicited by PLC-β1 that modulates Activator Protein-1 (AP-1) activity. Indeed, gel mobility shift assaysindicate that there is a c-jun binding site located in cyclin D3 promoter region specifically regulated byPLC-β1 and that c-jun binding activity is significantly increased by insulin stimulation and PLC-β1overexpression. Moreover, mutation of c-jun/AP-1 binding site decreases the basal cyclin D3 promoteractivity and eliminates its induction by insulin and PLC-β1 overexpression. Interestingly, we observedthat the ectopic expression of the Inositol Polyphosphate Multikinase (IPMK) in C2C12 myoblastsenhances cyclin D3 gene expression and that the mutation of c-jun site in cyclin D3 promoterdetermines an impairment of IPMK-dependent promoter induction. These results indicate that PLC-β1activates a c-jun/AP-1 target gene, i.e. cyclin D3, during myogenic differentiation through IPMKsignaling." @default.
- W72497253 created "2016-06-24" @default.
- W72497253 creator A5080207477 @default.
- W72497253 date "2008-05-26" @default.
- W72497253 modified "2023-09-24" @default.
- W72497253 title "RUOLO DELLA FOSFOLIPASI C-β1 NEL DIFFERENZIAMENTO MIOGENICO: IDENTIFICAZIONE DI NUOVI TARGET NUCLEARI" @default.
- W72497253 cites W1482441851 @default.
- W72497253 cites W1568965788 @default.
- W72497253 cites W1584157616 @default.
- W72497253 cites W1766228108 @default.
- W72497253 cites W1793858878 @default.
- W72497253 cites W1952579501 @default.
- W72497253 cites W1958742527 @default.
- W72497253 cites W1964733862 @default.
- W72497253 cites W1967202041 @default.
- W72497253 cites W1967336624 @default.
- W72497253 cites W1968336657 @default.
- W72497253 cites W1969655939 @default.
- W72497253 cites W1970692622 @default.
- W72497253 cites W1972877825 @default.
- W72497253 cites W1978274366 @default.
- W72497253 cites W1978649670 @default.
- W72497253 cites W1979118270 @default.
- W72497253 cites W1981182586 @default.
- W72497253 cites W1984769860 @default.
- W72497253 cites W1994071091 @default.
- W72497253 cites W1997739014 @default.
- W72497253 cites W1998129803 @default.
- W72497253 cites W1999250917 @default.
- W72497253 cites W2000932018 @default.
- W72497253 cites W2002127663 @default.
- W72497253 cites W2003160212 @default.
- W72497253 cites W2006455216 @default.
- W72497253 cites W2006499135 @default.
- W72497253 cites W2006739525 @default.
- W72497253 cites W2009772352 @default.
- W72497253 cites W2012012590 @default.
- W72497253 cites W2014716519 @default.
- W72497253 cites W2015226319 @default.
- W72497253 cites W2018901423 @default.
- W72497253 cites W2019434438 @default.
- W72497253 cites W2019673480 @default.
- W72497253 cites W2020674613 @default.
- W72497253 cites W2021682292 @default.
- W72497253 cites W2026494112 @default.
- W72497253 cites W2026579495 @default.
- W72497253 cites W2031666214 @default.
- W72497253 cites W2037838485 @default.
- W72497253 cites W2038512674 @default.
- W72497253 cites W2038612592 @default.
- W72497253 cites W2042127371 @default.
- W72497253 cites W2042326040 @default.
- W72497253 cites W2043860474 @default.
- W72497253 cites W2044502027 @default.
- W72497253 cites W2045194116 @default.
- W72497253 cites W2046122733 @default.
- W72497253 cites W2049074441 @default.
- W72497253 cites W2052250113 @default.
- W72497253 cites W2052651806 @default.
- W72497253 cites W2052866727 @default.
- W72497253 cites W2059749734 @default.
- W72497253 cites W2059829192 @default.
- W72497253 cites W2062748780 @default.
- W72497253 cites W2064034017 @default.
- W72497253 cites W2066121149 @default.
- W72497253 cites W2067488984 @default.
- W72497253 cites W2068011423 @default.
- W72497253 cites W2068132628 @default.
- W72497253 cites W2068488447 @default.
- W72497253 cites W2070811212 @default.
- W72497253 cites W2071737785 @default.
- W72497253 cites W2077666369 @default.
- W72497253 cites W2079853404 @default.
- W72497253 cites W2080419891 @default.
- W72497253 cites W2084828697 @default.
- W72497253 cites W2085593164 @default.
- W72497253 cites W2085693261 @default.
- W72497253 cites W2086036400 @default.
- W72497253 cites W2087358641 @default.
- W72497253 cites W2090133829 @default.
- W72497253 cites W2092249847 @default.
- W72497253 cites W2101103980 @default.
- W72497253 cites W2102476333 @default.
- W72497253 cites W2104433712 @default.
- W72497253 cites W2105728173 @default.
- W72497253 cites W2106468261 @default.
- W72497253 cites W2107844605 @default.
- W72497253 cites W2108666301 @default.
- W72497253 cites W2113221012 @default.
- W72497253 cites W2118224224 @default.
- W72497253 cites W2121628947 @default.
- W72497253 cites W2121808401 @default.
- W72497253 cites W2125457000 @default.
- W72497253 cites W2134971385 @default.
- W72497253 cites W2137140177 @default.
- W72497253 cites W2137479810 @default.
- W72497253 cites W2138752775 @default.
- W72497253 cites W2141985020 @default.
- W72497253 cites W2153232954 @default.
- W72497253 cites W2172205020 @default.